{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Husseiny MMAE"],"funding":["Vetenskapsrådet","Cancerfonden"],"pagination":["34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12220533"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["<h4>Background</h4>Human cells can synthesize methionine from homocysteine and folate-coupled methyl groups via the B<sub>12</sub>-dependent enzyme methionine synthase (MTR). Yet, it has been known for decades that cancer cells fail to grow when methionine is replaced by homocysteine, a phenomenon known as methionine dependence. The underlying mechanism remains unknown.<h4>Methods</h4>Cancer cell lines were cultured with homocysteine in place of methionine, and growth responses were measured. Revertant cells capable of growing in homocysteine were generated through long-term culture with high B<sub>12</sub> and analyzed using single-cell RNA-seq. Metabolite uptake/release was measured using isotope dilution and MTR activity was assessed using metabolic flux analysis (MFA). Functional rescu"],"journal":["Cancer & metabolism"],"pubmed_title":["The role of B&lt;sub&gt;12&lt;/sub&gt; deficiency and methionine synthase in methionine-dependent cancer cells."],"pmcid":["PMC12220533"],"funding_grant_id":["20 0974 PjF","2020-01631"],"pubmed_authors":["Husseiny MMAE","Nilsson R"],"additional_accession":[]},"is_claimable":false,"name":"The role of B&lt;sub&gt;12&lt;/sub&gt; deficiency and methionine synthase in methionine-dependent cancer cells.","description":"<h4>Background</h4>Human cells can synthesize methionine from homocysteine and folate-coupled methyl groups via the B<sub>12</sub>-dependent enzyme methionine synthase (MTR). Yet, it has been known for decades that cancer cells fail to grow when methionine is replaced by homocysteine, a phenomenon known as methionine dependence. The underlying mechanism remains unknown.<h4>Methods</h4>Cancer cell lines were cultured with homocysteine in place of methionine, and growth responses were measured. Revertant cells capable of growing in homocysteine were generated through long-term culture with high B<sub>12</sub> and analyzed using single-cell RNA-seq. Metabolite uptake/release was measured using isotope dilution and MTR activity was assessed using metabolic flux analysis (MFA). Functional rescu","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-07-15T08:59:39.948Z","creation":"2026-07-03T03:11:10.335Z"},"accession":"S-EPMC12220533","cross_references":{"pubmed":["40605016"],"doi":["10.1186/s40170-025-00405-2"]}}