{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Einenkel R"],"funding":["European Research Council","Human Frontier Science Program (HFSP)","Biotechnology and Biological Sciences Research Council"],"pagination":["1741-1757"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12221982"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(7)"],"pubmed_abstract":["The bacterial flagellum is essential for motility, adhesion and colonization in pathogens such as Salmonella enterica and Campylobacter jejuni. Its extracellular structure comprises the hook, hook-filament junction, filament and filament cap. Native structures of the hook-filament junction and the cap are lacking, and molecular mechanisms of cap-mediated filament assembly are largely uncharacterized. Here we use cryo-electron microscopy to resolve structures of the complete Salmonella extracellular flagellum including the pentameric FliD cap complex (3.7 Å) and the FlgKL hook-filament junction (2.9 Å), as well as the Campylobacter extracellular flagellum before filament assembly (6.5 Å). This, coupled with structure-guided mutagenesis and functional assays, reveals intermediates of filamen"],"journal":["Nature microbiology"],"pubmed_title":["The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation."],"pmcid":["PMC12221982"],"funding_grant_id":["BB/R009759/2","864971","RGY0080/2021","BB/V019732/1"],"pubmed_authors":["Gonzalez-Rodriguez N","Harrowell J","Qin K","Al-Otaibi NS","Einenkel R","Schmidt J","Erhardt M","Beeby M","Mann D","Bergeron JRC","Drobnic T","Cohen EJ","Shmakova E"],"additional_accession":[]},"is_claimable":false,"name":"The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation.","description":"The bacterial flagellum is essential for motility, adhesion and colonization in pathogens such as Salmonella enterica and Campylobacter jejuni. Its extracellular structure comprises the hook, hook-filament junction, filament and filament cap. Native structures of the hook-filament junction and the cap are lacking, and molecular mechanisms of cap-mediated filament assembly are largely uncharacterized. Here we use cryo-electron microscopy to resolve structures of the complete Salmonella extracellular flagellum including the pentameric FliD cap complex (3.7 Å) and the FlgKL hook-filament junction (2.9 Å), as well as the Campylobacter extracellular flagellum before filament assembly (6.5 Å). This, coupled with structure-guided mutagenesis and functional assays, reveals intermediates of filamen","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-06-01T15:32:13.875Z","creation":"2026-04-08T13:46:46.609Z"},"accession":"S-EPMC12221982","cross_references":{"pubmed":["40595287"],"doi":["10.1038/s41564-025-02037-0"]}}