biostudies-literatureUnknown358(7)Platella CThis study is supported by Università degli Studi di Milano: PSR2022_DIP_005_PI_CGIAN.The research leading to these results has received funding from: (i) Fondazione AIRC under IG 2020-ID.25046- P.I. Montesarchio Daniela; (ii) CN00000041 National Center for Gene Therapy and Drugs based on RNA Technology and the European Union-NextGeneration EU - P.I. Montesarchio Daniela; (iii) Federico II University, FRA2022 project (CUP: E65F22000050001) - P.I. Musumeci DomenicaThe synthesis and characterization of a mini-library of cyclic triimidazo triazine (TT) derivatives functionalized with one, two, or three ethynyl-N-methyl-pyridinium moieties are reported here. These compounds were designed with the aim of targeting cancer-related DNA G-quadruplex structures. The newly synthesized compounds were tested for their ability to bind G-quadruplexes from both telomeric and oncogene promoter sequences using an affinity chromatography-based assay, spectroscopic and electrophoretic techniques, as well as molecular docking analysis. The obtained results demonstrated the effective capacity of the investigated compounds to specifically recognize the selected G-quadruplex models, with their TT cores targeting the outer G-quartets and their positively charged N-methyl-pArchiv der Pharmaziee70037https://www.ebi.ac.uk/biostudies/studies/S-EPMC12223358biostudies-literatureCombining Cyclic Triimidazo Triazine Core With Ethynyl-N-Methyl-Pyridinium Groups for Targeting G-Quadruplex Structures.PMC12223358Malpicci DMusumeci DMontesarchio DDi Ciolo SGiannini CPlatella CLucenti EGaglione RArciello ACariati ECriscuolo AfalseCombining Cyclic Triimidazo Triazine Core With Ethynyl-N-Methyl-Pyridinium Groups for Targeting G-Quadruplex Structures.The synthesis and characterization of a mini-library of cyclic triimidazo triazine (TT) derivatives functionalized with one, two, or three ethynyl-N-methyl-pyridinium moieties are reported here. These compounds were designed with the aim of targeting cancer-related DNA G-quadruplex structures. The newly synthesized compounds were tested for their ability to bind G-quadruplexes from both telomeric and oncogene promoter sequences using an affinity chromatography-based assay, spectroscopic and electrophoretic techniques, as well as molecular docking analysis. The obtained results demonstrated the effective capacity of the investigated compounds to specifically recognize the selected G-quadruplex models, with their TT cores targeting the outer G-quartets and their positively charged N-methyl-p2025-01-01T00:00:00Z2025 Jul2026-06-02T05:59:49.401Z2026-04-15T03:09:56.097ZS-EPMC122233584060528110.1002/ardp.70037