biostudies-literatureUnknown241(8)Heuschkel MADeutsche ForschungsgemeinschaftHorizon 2020 Framework ProgrammeDeutsche Herzstiftung<h4>Aim</h4>Vascular calcification (VC), a characteristic feature of peripheral artery disease in patients with diabetes and chronic kidney disease, has been associated with poor prognosis. We hypothesize that hyperglycemia drives VC through alterations in metabolomic and transcriptomic profiles.<h4>Methods</h4>Human coronary artery smooth muscle cells (SMCs) were cultured with 0, 5.5, and 25 mM glucose under calcifying conditions. Untargeted metabolomic and transcriptomic analyses were performed at different time points. Mitochondrial respiration was examined using Seahorse analysis.<h4>Results</h4>Glucose-treated SMCs promoted extracellular matrix (ECM) calcification in a concentration- and time-dependent manner. The absence of glucose entirely abolished SMC calcification but reduced SMCActa physiologica (Oxford, England)e70075https://www.ebi.ac.uk/biostudies/studies/S-EPMC12230643biostudies-literatureHypotaurine Reduces Glucose-Mediated Vascular Calcification.PMC12230643Heuschkel MASchurgers LAdamski JJahnen-Dechent WArtati AGoettsch CGraber SMarx NJaminon AJankowski JfalseHypotaurine Reduces Glucose-Mediated Vascular Calcification.<h4>Aim</h4>Vascular calcification (VC), a characteristic feature of peripheral artery disease in patients with diabetes and chronic kidney disease, has been associated with poor prognosis. We hypothesize that hyperglycemia drives VC through alterations in metabolomic and transcriptomic profiles.<h4>Methods</h4>Human coronary artery smooth muscle cells (SMCs) were cultured with 0, 5.5, and 25 mM glucose under calcifying conditions. Untargeted metabolomic and transcriptomic analyses were performed at different time points. Mitochondrial respiration was examined using Seahorse analysis.<h4>Results</h4>Glucose-treated SMCs promoted extracellular matrix (ECM) calcification in a concentration- and time-dependent manner. The absence of glucose entirely abolished SMC calcification but reduced SMC2025-01-01T00:00:00Z2025 Aug2026-07-15T09:02:35.924Z2026-07-02T03:08:56.011ZS-EPMC122306434062003310.1111/apha.70075