biostudies-literatureUnknownWhite CMNINDS NIH HHSVisual perception appears largely stable in time. However, psychophysical studies have revealed that theta waves (4-8 Hz) can modulate perception and have been linked to various cognitive states and functions. Neither the contribution of theta waves to cortical activity nor their impact during aberrant brain states have been resolved at high spatiotemporal scales. Here, using cortex-wide population voltage imaging in awake mice, we found that bouts of 5-Hz oscillations in the visual cortex are accompanied by similar oscillations in the retrosplenial cortex, occurring both spontaneously and evoked by visual stimulation. Injection of psychotropic 5-HT2AR agonist induced a significant increase in spontaneous 5-Hz oscillations, and also increased the power, occurrence probability and temporal bioRxiv : the preprint server for biology2025.07.05.663288https://www.ebi.ac.uk/biostudies/studies/S-EPMC12236502biostudies-literaturePsychedelic 5-HT2A agonist increases spontaneous and evoked 5-Hz oscillations in visual and retrosplenial cortex.PMC12236502U01 NS099573Knopfel TStaadt RJancke DAzimi ZSong CWhite CMfalsePsychedelic 5-HT2A agonist increases spontaneous and evoked 5-Hz oscillations in visual and retrosplenial cortex.Visual perception appears largely stable in time. However, psychophysical studies have revealed that theta waves (4-8 Hz) can modulate perception and have been linked to various cognitive states and functions. Neither the contribution of theta waves to cortical activity nor their impact during aberrant brain states have been resolved at high spatiotemporal scales. Here, using cortex-wide population voltage imaging in awake mice, we found that bouts of 5-Hz oscillations in the visual cortex are accompanied by similar oscillations in the retrosplenial cortex, occurring both spontaneously and evoked by visual stimulation. Injection of psychotropic 5-HT2AR agonist induced a significant increase in spontaneous 5-Hz oscillations, and also increased the power, occurrence probability and temporal 2025-01-01T00:00:00Z2025 Jul2026-05-31T03:15:20.955Z2026-05-31T03:10:08.824ZS-EPMC122365024063116210.1101/2025.07.05.663288