{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Zhang M"],"pubmed_abstract":["<i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), the causal agent of wheat powdery mildew, poses a significant threat to global wheat production. As an obligate biotroph, <i>Bgt</i> is recalcitrant to stable genetic manipulation. Although host-induced gene silencing has been used for gene function studies, it remains ineffective for targeting genes active during pre-penetration stages. Consequently, the functional roles of many <i>Bgt</i> genes during pre-penetration stages remain largely unexplored. In this study, the feasibility of spray-induced gene silencing (SIGS) to characterize gene function during pre-penetration stages was evaluated. The results demonstrated that <i>Bgt</i> conidia and germ tubes efficiently took up environmental double-stranded RNA (dsRNA), enabling t"],"journal":["Frontiers in plant science"],"pagination":["1628068"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12239746"],"repository":["biostudies-literature"],"pubmed_title":["Spray-induced gene silencing enables the characterization of gene function during pre-penetration stages in &lt;i&gt;Blumeria graminis&lt;/i&gt; f. sp. &lt;i&gt;tritici&lt;/i&gt;."],"pmcid":["PMC12239746"],"pubmed_authors":["Zhou M","Li X","Wang A","Fan J","Jia Y","Zhang M","Liu T","Zhou Y","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Spray-induced gene silencing enables the characterization of gene function during pre-penetration stages in &lt;i&gt;Blumeria graminis&lt;/i&gt; f. sp. &lt;i&gt;tritici&lt;/i&gt;.","description":"<i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), the causal agent of wheat powdery mildew, poses a significant threat to global wheat production. As an obligate biotroph, <i>Bgt</i> is recalcitrant to stable genetic manipulation. Although host-induced gene silencing has been used for gene function studies, it remains ineffective for targeting genes active during pre-penetration stages. Consequently, the functional roles of many <i>Bgt</i> genes during pre-penetration stages remain largely unexplored. In this study, the feasibility of spray-induced gene silencing (SIGS) to characterize gene function during pre-penetration stages was evaluated. The results demonstrated that <i>Bgt</i> conidia and germ tubes efficiently took up environmental double-stranded RNA (dsRNA), enabling t","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-07-15T10:54:50.7Z","creation":"2026-07-03T03:15:43.649Z"},"accession":"S-EPMC12239746","cross_references":{"pubmed":["40636020"],"doi":["10.3389/fpls.2025.1628068"]}}