biostudies-literatureUnknown59Duan HColorectal cancer (CRC) is among top most common cancers with high mortality rate and major health issue yet to resolve across the globe. miR-23b is an oncogene in many cancers including CRC. The route of involvement of miR-23b in the regulation of CRC is still unclear. Study explored bioregulatory mechanism of miR-23b against CRC involving in vitro and animal model experimentation via experimentation involving ki67 immunohistochemistry, CCK8, EDU, and Transwell cell migration. The bioinformatic studies were employed to predict miR-23b target tumor suppressor PTPRG while the luciferase reporter gene assay was used to find the binding capabilities of miR-23b to the 3'-UTR of PTPRG. The changes in the expression of miR-23b were found in three CRC cell lines including SW480, HT29, and Caco2, Translational oncology102447https://www.ebi.ac.uk/biostudies/studies/S-EPMC12240168biostudies-literaturemiR-23b is a negative regulator of tumor suppressing PTPRG in colorectal cancer.PMC12240168Yang JHe CWang TLi FHu HZhai KZhang SKhan GJDuan HJia JWang LfalsemiR-23b is a negative regulator of tumor suppressing PTPRG in colorectal cancer.Colorectal cancer (CRC) is among top most common cancers with high mortality rate and major health issue yet to resolve across the globe. miR-23b is an oncogene in many cancers including CRC. The route of involvement of miR-23b in the regulation of CRC is still unclear. Study explored bioregulatory mechanism of miR-23b against CRC involving in vitro and animal model experimentation via experimentation involving ki67 immunohistochemistry, CCK8, EDU, and Transwell cell migration. The bioinformatic studies were employed to predict miR-23b target tumor suppressor PTPRG while the luciferase reporter gene assay was used to find the binding capabilities of miR-23b to the 3'-UTR of PTPRG. The changes in the expression of miR-23b were found in three CRC cell lines including SW480, HT29, and Caco2, 2025-01-01T00:00:00Z2025 Sep2026-03-15T14:00:58.242Z2025-08-13T03:04:29.089ZS-EPMC122401684056179810.1016/j.tranon.2025.102447