{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Kim SH"],"pubmed_abstract":["<h4>Introduction</h4>Abemaciclib, a CDK4/6 inhibitor, is well-established for treating hormone receptor-positive and HER2-negative (HR<sup>+</sup>/HER2<sup>-</sup>) human breast cancer by inducing G1 cell cycle arrest. However, its antitumor effects in other cancers, including canine melanoma, remain largely unexplored. Canine melanoma often harbors CDK4/6 copy number gains and cell cycle dysregulation, suggesting it may be a suitable target for abemaciclib.<h4>Methods</h4>Five canine melanoma cell lines (CMeC1, KMeC, LMeC, UCDK9M4, and UCDK9M5) were used to evaluate the antitumor effects of abemaciclib. Cell viability and migration were assessed using Cell Counting Kit-8 and wound healing assays, respectively. Cell cycle distribution was analyzed by flow cytometry, and the expression of c"],"journal":["Frontiers in veterinary science"],"pagination":["1603686"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12240792"],"repository":["biostudies-literature"],"pubmed_title":["Abemaciclib induces G1 arrest and lysosomal dysfunction in canine melanoma cells: synergistic effects with fenbendazole."],"pmcid":["PMC12240792"],"pubmed_authors":["Kim SH","Song KH","Choi JY","Ryu MO","Suh YH","Seo KW"],"additional_accession":[]},"is_claimable":false,"name":"Abemaciclib induces G1 arrest and lysosomal dysfunction in canine melanoma cells: synergistic effects with fenbendazole.","description":"<h4>Introduction</h4>Abemaciclib, a CDK4/6 inhibitor, is well-established for treating hormone receptor-positive and HER2-negative (HR<sup>+</sup>/HER2<sup>-</sup>) human breast cancer by inducing G1 cell cycle arrest. However, its antitumor effects in other cancers, including canine melanoma, remain largely unexplored. Canine melanoma often harbors CDK4/6 copy number gains and cell cycle dysregulation, suggesting it may be a suitable target for abemaciclib.<h4>Methods</h4>Five canine melanoma cell lines (CMeC1, KMeC, LMeC, UCDK9M4, and UCDK9M5) were used to evaluate the antitumor effects of abemaciclib. Cell viability and migration were assessed using Cell Counting Kit-8 and wound healing assays, respectively. Cell cycle distribution was analyzed by flow cytometry, and the expression of c","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-07-15T12:48:26.633Z","creation":"2026-07-04T03:16:11.576Z"},"accession":"S-EPMC12240792","cross_references":{"pubmed":["40642276"],"doi":["10.3389/fvets.2025.1603686"]}}