{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Winkelman JT"],"pubmed_abstract":["Bacteria employ sophisticated post-transcriptional regulatory mechanisms to adapt to environmental changes. Carbon storage regulator A (CsrA), a highly conserved small RNA-binding protein, serves as a critical post-transcriptional regulator by recognizing GGA motifs in target transcripts and typically repressing translation. Despite extensive research, how this conserved regulator has evolved diverse species-specific regulatory networks remains unclear. Here, using <i>Bacillus subtilis</i> as an experimental chassis, we have developed Swarm-seq - a high-throughput functional genomics platform capable of assessing CsrA homologs across the Bacterial domain for their ability to regulate flagella-dependent swarming motility. Systematically testing over five-hundred codon-optimized <i>csrA</i> "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.05.05.652322"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12247646"],"repository":["biostudies-literature"],"pubmed_title":["Swarm-seq Reveals Functional Divergence in CsrA Homologs Across Bacteria."],"pmcid":["PMC12247646"],"pubmed_authors":["Winkelman JT","Winkelman JD","Yarberry E","Mukherjee S"],"additional_accession":[]},"is_claimable":false,"name":"Swarm-seq Reveals Functional Divergence in CsrA Homologs Across Bacteria.","description":"Bacteria employ sophisticated post-transcriptional regulatory mechanisms to adapt to environmental changes. Carbon storage regulator A (CsrA), a highly conserved small RNA-binding protein, serves as a critical post-transcriptional regulator by recognizing GGA motifs in target transcripts and typically repressing translation. Despite extensive research, how this conserved regulator has evolved diverse species-specific regulatory networks remains unclear. Here, using <i>Bacillus subtilis</i> as an experimental chassis, we have developed Swarm-seq - a high-throughput functional genomics platform capable of assessing CsrA homologs across the Bacterial domain for their ability to regulate flagella-dependent swarming motility. Systematically testing over five-hundred codon-optimized <i>csrA</i> ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2025-08-28T03:09:23.45Z","creation":"2025-08-28T03:09:23.45Z"},"accession":"S-EPMC12247646","cross_references":{"pubmed":["40654832"],"doi":["10.1101/2025.05.05.652322"]}}