biostudies-literatureUnknownTazin SNICHD NIH HHSSoil floors are common in low-income countries and can harbor contamination from unsafely managed human and animal fecal waste. Soil/dust ingestion directly from floors or indirectly via hands, drinking water and food can significantly contribute to children's ingestion of fecal organisms. We assessed if finished (e.g., concrete) floors are associated with lower <i>E. coli</i> contamination in the domestic environment in rural Bangladesh. We collected samples from 1864 households over 3.5 years, including stored drinking water, child and caregiver hand rinses, courtyard soil, food, and flies (n=24,118 samples), and enumerated <i>E. coli</i> using IDEXX Quanti-Tray/2000. Controlling for potential confounders (socio-demographics, water/sanitation status, animal ownership), households with fibioRxiv : the preprint server for biology2025.05.07.652756https://www.ebi.ac.uk/biostudies/studies/S-EPMC12247773biostudies-literatureAssociations between floor material and &lt;i&gt;E. coli&lt;/i&gt; contamination in rural Bangladeshi households.PMC12247773R01 HD078912Hubbard AELuby SPSen DIslam SKwong LHNiven CArnold BFPickering AJRahman MAlam MErcumen ABenjamin-Chung JColford JMMertens AIslam MUnicomb LTazin SfalseAssociations between floor material and &lt;i&gt;E. coli&lt;/i&gt; contamination in rural Bangladeshi households.Soil floors are common in low-income countries and can harbor contamination from unsafely managed human and animal fecal waste. Soil/dust ingestion directly from floors or indirectly via hands, drinking water and food can significantly contribute to children's ingestion of fecal organisms. We assessed if finished (e.g., concrete) floors are associated with lower <i>E. coli</i> contamination in the domestic environment in rural Bangladesh. We collected samples from 1864 households over 3.5 years, including stored drinking water, child and caregiver hand rinses, courtyard soil, food, and flies (n=24,118 samples), and enumerated <i>E. coli</i> using IDEXX Quanti-Tray/2000. Controlling for potential confounders (socio-demographics, water/sanitation status, animal ownership), households with fi2025-01-01T00:00:00Z2025 May2025-08-27T03:07:04.661Z2025-08-27T03:07:04.661ZS-EPMC122477734065492210.1101/2025.05.07.652756