biostudies-literatureUnknownSheth UNINDS NIH HHS<h4>Objectives</h4>Neurofilament light (NfL) is an established biofluid marker of neuroaxonal injury for neurological diseases. Several high-throughput and sensitive immunoassays have been developed to quantify NfL in blood and cerebrospinal fluid (CSF), facilitating the use of NfL as a biomarker in research and clinical practice. However, because of the lack of rigorous comparisons of assays, it has been difficult to determine whether data are comparable and whether assay performance differs. Here, we compared the performance of five NfL immunoassays.<h4>Methods</h4>To assess the five NfL immunoassays (Fujirebio, ProteinSimple, Quanterix, Roche and Siemens), we used pooled plasma or pooled CSF, as well as unique samples from 20 healthy controls and 20 individuals with El Escorial defined bioRxiv : the preprint server for biology2025.05.05.652212https://www.ebi.ac.uk/biostudies/studies/S-EPMC12247930biostudies-literatureMeasuring neurofilament light in human plasma and cerebrospinal fluid: a comparison of five analytical immunoassays.PMC12247930U54 NS092091U01 NS107027Harrison RFerber KBenatar MRosenbaugh EGGraham DKhillan RHarris GAZetterberg HBjorklund NLGendron TFLiu YDi Daniel ESheth UBevis AKahn OIMitic LLfalseMeasuring neurofilament light in human plasma and cerebrospinal fluid: a comparison of five analytical immunoassays.<h4>Objectives</h4>Neurofilament light (NfL) is an established biofluid marker of neuroaxonal injury for neurological diseases. Several high-throughput and sensitive immunoassays have been developed to quantify NfL in blood and cerebrospinal fluid (CSF), facilitating the use of NfL as a biomarker in research and clinical practice. However, because of the lack of rigorous comparisons of assays, it has been difficult to determine whether data are comparable and whether assay performance differs. Here, we compared the performance of five NfL immunoassays.<h4>Methods</h4>To assess the five NfL immunoassays (Fujirebio, ProteinSimple, Quanterix, Roche and Siemens), we used pooled plasma or pooled CSF, as well as unique samples from 20 healthy controls and 20 individuals with El Escorial defined 2025-01-01T00:00:00Z2025 May2025-08-17T03:06:10.807Z2025-08-17T03:06:10.807ZS-EPMC122479304065495710.1101/2025.05.05.652212