{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Liu Y"],"funding":["NIGMS NIH HHS","NIH HHS"],"pubmed_abstract":["MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression of target messenger (m) RNAs. To maintain proper miRNA expression levels, the enzymatic processing of primary and precursor miRNA elements must be strictly controlled. However, the molecular determinants underlying this strict regulation of miRNA biogenesis are not fully understood. Here, we determined the solution structure of pre-miR-20a, an oncogenic miRNA and component of the oncomiR-1 cluster, using nuclear magnetic spectroscopy (NMR) spectroscopy and small angle X-ray scattering (SAXS). Our structural studies informed on key secondary structure elements of pre-miR-20a which may control its enzymatic processing, namely a flexible apical loop and single-nucleotide bulge near the dicing sit"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.05.07.652689"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12247985"],"repository":["biostudies-literature"],"pubmed_title":["Structural features within precursor microRNA-20a regulate Dicer-TRBP processing."],"pmcid":["PMC12247985"],"funding_grant_id":["P30 GM138395","R35 GM138279","S10 OD018090"],"pubmed_authors":["Munsayac A","Liu Y","Keane SC","Harkner CT","Westwood MN"],"additional_accession":[]},"is_claimable":false,"name":"Structural features within precursor microRNA-20a regulate Dicer-TRBP processing.","description":"MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression of target messenger (m) RNAs. To maintain proper miRNA expression levels, the enzymatic processing of primary and precursor miRNA elements must be strictly controlled. However, the molecular determinants underlying this strict regulation of miRNA biogenesis are not fully understood. Here, we determined the solution structure of pre-miR-20a, an oncogenic miRNA and component of the oncomiR-1 cluster, using nuclear magnetic spectroscopy (NMR) spectroscopy and small angle X-ray scattering (SAXS). Our structural studies informed on key secondary structure elements of pre-miR-20a which may control its enzymatic processing, namely a flexible apical loop and single-nucleotide bulge near the dicing sit","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2025-08-23T03:08:50.335Z","creation":"2025-08-23T03:08:50.335Z"},"accession":"S-EPMC12247985","cross_references":{"pubmed":["40654793"],"doi":["10.1101/2025.05.07.652689"]}}