biostudies-literatureUnknown7(16)Dave RThis study expands the platform for amyloidogenic building blocks, such as nucleobases, and their self-assembly. Here, we examine the self-assembly profile of nucleobases such as guanine, cytosine, and thymine and determine that these nucleobases, while aged, produce small globules which gradually transform into fibrillar assemblies. Notably, the amyloid-like fibrillation in adenine and uracil has already been reported; hence, it was imperative to understand the amyloidogenic propensity in these unexplored nucleobases. The aggregates formed by guanine, cytosine, and thymine interestingly reveal a distinctive spectrum characteristic of amyloidogenic proteins after binding to the amyloid-specific dye Thioflavin T (ThT). The MTT assay in human retinal pigment epithelial RPE-1 cell lines reveaNanoscale advances5093-5103https://www.ebi.ac.uk/biostudies/studies/S-EPMC12258404biostudies-literatureNucleobase self-assembly: aggregation, morphological characterization, and toxicity analysis.PMC12258404Verma SJaiswal ANazir ANaseer AGour NDave RSingh ABhatia DPatel MfalseNucleobase self-assembly: aggregation, morphological characterization, and toxicity analysis.This study expands the platform for amyloidogenic building blocks, such as nucleobases, and their self-assembly. Here, we examine the self-assembly profile of nucleobases such as guanine, cytosine, and thymine and determine that these nucleobases, while aged, produce small globules which gradually transform into fibrillar assemblies. Notably, the amyloid-like fibrillation in adenine and uracil has already been reported; hence, it was imperative to understand the amyloidogenic propensity in these unexplored nucleobases. The aggregates formed by guanine, cytosine, and thymine interestingly reveal a distinctive spectrum characteristic of amyloidogenic proteins after binding to the amyloid-specific dye Thioflavin T (ThT). The MTT assay in human retinal pigment epithelial RPE-1 cell lines revea2025-01-01T00:00:00Z2025 Aug2026-03-31T11:48:54.044Z2025-09-01T03:05:54.375ZS-EPMC122584044066660510.1039/d5na00259a