{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Carr JF"],"pubmed_abstract":["In gram-negative bacteria, the outer membrane (OM) acts in conjunction with the peptidoglycan (PG) cell wall as a barrier against physical, osmotic, and toxic environmental stresses including antibiotics. SanA, an inner membrane protein in <i>Escherichia coli</i> K-12, is required for vancomycin resistance at high temperatures (>42 °C) and we have previously demonstrated that SanA impacts sodium dodecyl sulfate (SDS) resistance during stationary phase reached from carbon limitation. However, its function remains unknown. Here, we show that Δ<i>sanA</i> has a synthetic genetic interaction with Δ<i>wecA</i>, a mutation increasing the availability of the isoprenoid carrier for PG biosynthesis. Specifically, the Δ<i>sanA</i> Δ<i>wecA</i> strain demonstrated heightened SDS EDTA sensitivity, Rcs"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.06.10.658892"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12259093"],"repository":["biostudies-literature"],"pubmed_title":["Impaired envelope integrity in the absence of SanA is linked to increased Lipid II availability and an imbalance of FtsI and FtsW activities."],"pmcid":["PMC12259093"],"pubmed_authors":["De Santiago CB","Bhut SA","Mitchell AM","Herrera CM","Nan B","Robert Wei","Carr JF","Stephen Trent M","Warzecha DJ"],"additional_accession":[]},"is_claimable":false,"name":"Impaired envelope integrity in the absence of SanA is linked to increased Lipid II availability and an imbalance of FtsI and FtsW activities.","description":"In gram-negative bacteria, the outer membrane (OM) acts in conjunction with the peptidoglycan (PG) cell wall as a barrier against physical, osmotic, and toxic environmental stresses including antibiotics. SanA, an inner membrane protein in <i>Escherichia coli</i> K-12, is required for vancomycin resistance at high temperatures (>42 °C) and we have previously demonstrated that SanA impacts sodium dodecyl sulfate (SDS) resistance during stationary phase reached from carbon limitation. However, its function remains unknown. Here, we show that Δ<i>sanA</i> has a synthetic genetic interaction with Δ<i>wecA</i>, a mutation increasing the availability of the isoprenoid carrier for PG biosynthesis. Specifically, the Δ<i>sanA</i> Δ<i>wecA</i> strain demonstrated heightened SDS EDTA sensitivity, Rcs","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2025-08-13T03:04:38.907Z","creation":"2025-08-13T03:04:38.907Z"},"accession":"S-EPMC12259093","cross_references":{"pubmed":["40661497"],"doi":["10.1101/2025.06.10.658892"]}}