biostudies-literatureUnknown75(5)Zhu M<h4>Aim</h4>The interaction between dental pulp stem cells (DPSCs) and vascular endothelial cells (ECs) is crucial to the speedy establishment of functional blood circulation within the transplanted pulp tissue. It is a complex process involving direct cell contact and paracrine signalling. The transmembrane domains of α1-Na⁺/K⁺-ATPase (ATP1A1) have been shown to influence tumour angiogenesis. Its role in regulating DPSCs/ECs interaction in vascular formation remains unknown. This study aimed to explore ATP1A1 on DPSCs/ECs communication, vascular network formation, and underlying mechanisms.<h4>Methods</h4>The formation of vessel structures within different culture systems was examined. The expression of pericyte-like markers and Na⁺/K⁺-ATPase-reInternational dental journal100870https://www.ebi.ac.uk/biostudies/studies/S-EPMC12260422biostudies-literatureATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity.PMC12260422Zhu MWu BJiang SZhang CZou TfalseATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity.<h4>Aim</h4>The interaction between dental pulp stem cells (DPSCs) and vascular endothelial cells (ECs) is crucial to the speedy establishment of functional blood circulation within the transplanted pulp tissue. It is a complex process involving direct cell contact and paracrine signalling. The transmembrane domains of α1-Na⁺/K⁺-ATPase (ATP1A1) have been shown to influence tumour angiogenesis. Its role in regulating DPSCs/ECs interaction in vascular formation remains unknown. This study aimed to explore ATP1A1 on DPSCs/ECs communication, vascular network formation, and underlying mechanisms.<h4>Methods</h4>The formation of vessel structures within different culture systems was examined. The expression of pericyte-like markers and Na⁺/K⁺-ATPase-re2025-01-01T00:00:00Z2025 Jul2026-03-31T10:27:38.204Z2025-08-23T03:09:16.24ZS-EPMC122604224062820210.1016/j.identj.2025.100870