{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Low TCH"],"funding":["NIGMS NIH HHS"],"pubmed_abstract":["Post-transcriptional regulation is particularly prominent during the maternal-to-zygotic transition (MZT), a developmental phase during which a large proportion of maternally provided mRNAs is repressed and cleared from metazoan embryos. RNA-binding proteins (RBPs) are key components of the post-transcriptional regulatory machinery. We show that the ORB2 RBP, the Drosophila ortholog of human Cytoplasmic Polyadenylation Element Binding Protein (hCPEB) 2-4 protein subfamily, binds to hundreds of maternally provided, rare-codon-enriched mRNAs in early embryos; that ORB2 targets are translationally repressed and unstable during the MZT; identify a U-rich motif enriched in ORB2 targets' 3'UTRs; and show that this motif confers ORB2 binding and repression to a luciferase reporter mRNA in S2 tiss"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.07.10.664187"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12265677"],"repository":["biostudies-literature"],"pubmed_title":["The ORB2 RNA-binding protein negatively regulates its target transcripts during the Drosophila maternal-to-zygotic transition via its functionally conserved Zinc-binding 'ZZ' domain."],"pmcid":["PMC12265677"],"funding_grant_id":["R01 GM129325"],"pubmed_authors":["Weerasooriya C","Lin S","Luo H","Smibert CA","Low TCH","Wang Z","Karaiskakis A","Angers S","Lipshitz HD"],"additional_accession":[]},"is_claimable":false,"name":"The ORB2 RNA-binding protein negatively regulates its target transcripts during the Drosophila maternal-to-zygotic transition via its functionally conserved Zinc-binding 'ZZ' domain.","description":"Post-transcriptional regulation is particularly prominent during the maternal-to-zygotic transition (MZT), a developmental phase during which a large proportion of maternally provided mRNAs is repressed and cleared from metazoan embryos. RNA-binding proteins (RBPs) are key components of the post-transcriptional regulatory machinery. We show that the ORB2 RBP, the Drosophila ortholog of human Cytoplasmic Polyadenylation Element Binding Protein (hCPEB) 2-4 protein subfamily, binds to hundreds of maternally provided, rare-codon-enriched mRNAs in early embryos; that ORB2 targets are translationally repressed and unstable during the MZT; identify a U-rich motif enriched in ORB2 targets' 3'UTRs; and show that this motif confers ORB2 binding and repression to a luciferase reporter mRNA in S2 tiss","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-07-03T03:24:56.096Z","creation":"2026-07-03T03:15:37.056Z"},"accession":"S-EPMC12265677","cross_references":{"pubmed":["40672220"],"doi":["10.1101/2025.07.10.664187"]}}