biostudies-literatureMcDaniel JHUnited States Department of Commerce | National Institute of Standards and Technology (NIST)NIA NIH HHSU.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (NHGRI)NHGRI NIH HHSNational Science Foundation (NSF)NCI NIH HHSU.S. Department of Health & Human Services | National Institutes of Health (NIH)NIGMS NIH HHSNIH HHS1195https://www.ebi.ac.uk/biostudies/studies/S-EPMC12267650biostudies-literatureUnknown12(1)The Genome in a Bottle Consortium (GIAB), hosted by the National Institute of Standards and Technology (NIST), is developing new matched tumor-normal samples, the first explicitly consented for public dissemination of genomic data and cell lines. Here, we describe a comprehensive genomic dataset from the first individual, HG008, including DNA from an adherent, epithelial-like pancreatic ductal adenocarcinoma (PDAC) tumor cell line and matched normal cells from duodenal and pancreatic tissues. Data for the tumor-normal matched samples comes from seventeen distinct state-of-the-art whole genome measurement technologies, including high depth short and long-read bulk whole genome sequencing (WGS), single cell WGS, Hi-C, and karyotyping. These data will be used by the GIAB Consortium to developScientific dataDevelopment and extensive sequencing of a broadly-consented Genome in a Bottle matched tumor-normal pair.PMC12267650R01 CA266279U01 CA25340560NANB20D166R35GM133747OT2OD034190OT2 OD034190R35 GM133747R01 HG011274U24 HG011853R01HG011274U24HG011853S10 OD028587P01 CA272295U54 AG0893341936791U01CA2534051919691Shelton JAkeson SPaten BAhmad TLambert CMehta HSteinsnyder ZKrieger GHusar GRemington JProszynski JCole KDSudkamp MHirschberg JWCatreux SPark JIremadze NJain MBhakta MSPatel VViolich IWagner JSikkink KRojas AScheffler KLajoie BRHannah LCKennedy KWenger AMEacker SHe ZNarzisi GRosen GLBaybayan PLevy SMcLaughlin IJTruong SHe HJSedlazeck FJMcDaniel JHSanborn JZYu HCJhangiani SNBryant AMcNulty BSalas-Gonzalez ILipson DThibaud-Nissen FWiseman KVernich MWescott MCross EMunding EMBehera SHastie ARKeskus AGPaulin LFSoifer IDoddapaneni HTrow JGross SMiga KHMuzny DMBenjamin HMason CEOlson NDReeves CKolmogorov MGingras MCKingan SBGardner JGooden AAKingsley CWeigman VSayyari EBrundu FKim YMcCoy RLiss ASZook JMRyon KXiao CSchmitt AAdamson AWood MfalseDevelopment and extensive sequencing of a broadly-consented Genome in a Bottle matched tumor-normal pair.The Genome in a Bottle Consortium (GIAB), hosted by the National Institute of Standards and Technology (NIST), is developing new matched tumor-normal samples, the first explicitly consented for public dissemination of genomic data and cell lines. Here, we describe a comprehensive genomic dataset from the first individual, HG008, including DNA from an adherent, epithelial-like pancreatic ductal adenocarcinoma (PDAC) tumor cell line and matched normal cells from duodenal and pancreatic tissues. Data for the tumor-normal matched samples comes from seventeen distinct state-of-the-art whole genome measurement technologies, including high depth short and long-read bulk whole genome sequencing (WGS), single cell WGS, Hi-C, and karyotyping. These data will be used by the GIAB Consortium to develop2025-01-01T00:00:00Z2025 Jul2026-05-01T18:37:00.508Z2026-04-07T17:09:00.448ZS-EPMC122676504067038610.1038/s41597-025-05438-2