biostudies-literatureUnknown51Wu YThe expanding global population intensifies demand for sustainable protein sources. Cell-cultured meat (CM) offers a promising alternative to conventional meat production but faces challenges in scalability and food-grade scaffold design. Current scaffolds often fail to replicate muscle tissue's structural and mechanical properties or support large-scale CM production. Moreover, the sensory and nutritional qualities of CM remain understudied. Here, we developed a novel lotus fiber-based natural plant fiber (NPF) scaffold mimicking native muscle tissue architecture. Porcine muscle stem cells (pMuSCs) were cultured on the NPF scaffold (pMuSCs-NPF), and their viability, proliferation, and differentiation were evaluated. The NPF scaffolds exhibited high biocompatibility and promoted pMuSCs aliBioactive materials807-824https://www.ebi.ac.uk/biostudies/studies/S-EPMC12268086biostudies-literatureLotus fiber-derived scaffolds for enhanced cultured meat production: Quality and sustainability.PMC12268086Tang JLi YHe CShi LYang QZhang CDai JWu YfalseLotus fiber-derived scaffolds for enhanced cultured meat production: Quality and sustainability.The expanding global population intensifies demand for sustainable protein sources. Cell-cultured meat (CM) offers a promising alternative to conventional meat production but faces challenges in scalability and food-grade scaffold design. Current scaffolds often fail to replicate muscle tissue's structural and mechanical properties or support large-scale CM production. Moreover, the sensory and nutritional qualities of CM remain understudied. Here, we developed a novel lotus fiber-based natural plant fiber (NPF) scaffold mimicking native muscle tissue architecture. Porcine muscle stem cells (pMuSCs) were cultured on the NPF scaffold (pMuSCs-NPF), and their viability, proliferation, and differentiation were evaluated. The NPF scaffolds exhibited high biocompatibility and promoted pMuSCs ali2025-01-01T00:00:00Z2025 Sep2026-03-27T15:54:27.535Z2025-08-24T03:09:05.927ZS-EPMC122680864067826210.1016/j.bioactmat.2025.06.048