{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kefalopoulou S"],"funding":["EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 Marie Skłodowska-Curie Actions (H2020 Excellent Science - Marie Skłodowska-Curie Actions)","Swiss National Science Foundation","European Research Council","Dutch Research Council (NWO)","Human Frontier Science Program (HFSP)"],"pagination":["1186-1198"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12270907"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(7)"],"pubmed_abstract":["The regulation of gene expression is governed at multiple levels of chromatin organization. However, how gene regulation is co-ordinated remains relatively unexplored. Here we develop Dam&ChIC, a method that enables retrospective and multifactorial chromatin profiling in single cells. Dam&ChIC employs chromatin labelling in living cells with <sup>m6</sup>A to acquire a past chromatin state, coupled with an antibody-mediated readout to capture the present chromatin state. Analyses of diverse factor combinations highlight its versatility and superior resolution. By tracking lamina-associated domain inheritance over the cell cycle, we showcase that Dam&ChIC provides retrospective single-cell chromatin data. When applied in random X chromosome inactivation, Dam&ChIC disentangles the temporal o"],"journal":["Nature cell biology"],"pubmed_title":["Retrospective and multifactorial single-cell profiling reveals sequential chromatin reorganization during X inactivation."],"pmcid":["PMC12270907"],"funding_grant_id":["184.034.019","798573","016.161.339","NWO-CW714.016.001","101053581","174991","LT000209/2018-L"],"pubmed_authors":["Kefalopoulou S","Korthout T","Zeller P","van Oudenaarden A","Kind J","Rullens PMJ","de Vries SS","de Luca KL"],"additional_accession":[]},"is_claimable":false,"name":"Retrospective and multifactorial single-cell profiling reveals sequential chromatin reorganization during X inactivation.","description":"The regulation of gene expression is governed at multiple levels of chromatin organization. However, how gene regulation is co-ordinated remains relatively unexplored. Here we develop Dam&ChIC, a method that enables retrospective and multifactorial chromatin profiling in single cells. Dam&ChIC employs chromatin labelling in living cells with <sup>m6</sup>A to acquire a past chromatin state, coupled with an antibody-mediated readout to capture the present chromatin state. Analyses of diverse factor combinations highlight its versatility and superior resolution. By tracking lamina-associated domain inheritance over the cell cycle, we showcase that Dam&ChIC provides retrospective single-cell chromatin data. When applied in random X chromosome inactivation, Dam&ChIC disentangles the temporal o","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-07-15T10:55:52.254Z","creation":"2025-08-14T03:05:38.43Z"},"accession":"S-EPMC12270907","cross_references":{"pubmed":["40640501"],"doi":["10.1038/s41556-025-01687-w"]}}