biostudies-literatureUnknown7(3)Li XSince the discovery of the double helix structure of deoxyribonucleic acid (DNA), significant progress has been made in engineering technologies such as gene analysis and editing, greatly promoting the development of biomedical research and clinical applications. In recent years, the rapid development of genome-editing technologies, especially the clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) system, have brought unprecedented opportunities for biomedical research and clinical applications. However, with the widespread application of genome-editing technologies, biosafety issues have gradually attracted the attention of the scientific community and the public. Potential risks such as off-target effects, genomic instability, and ethical and legBiosafety and health141-151https://www.ebi.ac.uk/biostudies/studies/S-EPMC12276576biostudies-literatureBiosafety considerations triggered by genome-editing technologies.PMC12276576Yao LLi XXie YZhang ZCao WWang SGao YGao ZWei XDeng WLi MfalseBiosafety considerations triggered by genome-editing technologies.Since the discovery of the double helix structure of deoxyribonucleic acid (DNA), significant progress has been made in engineering technologies such as gene analysis and editing, greatly promoting the development of biomedical research and clinical applications. In recent years, the rapid development of genome-editing technologies, especially the clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) system, have brought unprecedented opportunities for biomedical research and clinical applications. However, with the widespread application of genome-editing technologies, biosafety issues have gradually attracted the attention of the scientific community and the public. Potential risks such as off-target effects, genomic instability, and ethical and leg2025-01-01T00:00:00Z2025 Jun2026-03-16T07:44:12.351Z2025-08-15T03:06:18.519ZS-EPMC122765764069303610.1016/j.bsheal.2025.05.003