{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Cai YG"],"pubmed_abstract":["This study investigated the metabolic profiles of two isomeric psychoactive agents, methyl-ketamine [2-(ortho-tolyl)-2-(methylamino)cyclohexanone] and 2-oxo-PCE [2-(phenyl)-2-(ethylamino)cyclohexanone], in rats. Following oral administration, blood, liver, and urine samples were collected at timed intervals and analyzed via ultrahigh performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). Metabolomic comparisons revealed distinct metabolic pathways driven by structural differences. Methyl-ketamine primarily underwent cyclohexanone hydroxylation, dehydration, N-demethylation, cyclohexanone carbonyl hydrogenation, and glucuronidation, with N-dealkylation as the dominant process. In contrast, 2-oxo-PCE metabolism involved carbonyl hydrogenation of cycloh"],"journal":["Forensic science international. Synergy"],"pagination":["100623"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12281064"],"repository":["biostudies-literature"],"pubmed_title":["Metabolic patterns of new psychoactive substances: Methyl-ketamine and 2-oxo-PCE in rats using UHPLC-QTOF analysis."],"pmcid":["PMC12281064"],"pubmed_authors":["Wu YF","Wang QH","Cai YG","Feng JY","Dai Y","Wang YJ","Mo Y"],"additional_accession":[]},"is_claimable":false,"name":"Metabolic patterns of new psychoactive substances: Methyl-ketamine and 2-oxo-PCE in rats using UHPLC-QTOF analysis.","description":"This study investigated the metabolic profiles of two isomeric psychoactive agents, methyl-ketamine [2-(ortho-tolyl)-2-(methylamino)cyclohexanone] and 2-oxo-PCE [2-(phenyl)-2-(ethylamino)cyclohexanone], in rats. Following oral administration, blood, liver, and urine samples were collected at timed intervals and analyzed via ultrahigh performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). Metabolomic comparisons revealed distinct metabolic pathways driven by structural differences. Methyl-ketamine primarily underwent cyclohexanone hydroxylation, dehydration, N-demethylation, cyclohexanone carbonyl hydrogenation, and glucuronidation, with N-dealkylation as the dominant process. In contrast, 2-oxo-PCE metabolism involved carbonyl hydrogenation of cycloh","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-03-27T16:22:36.725Z","creation":"2025-08-31T03:05:15.794Z"},"accession":"S-EPMC12281064","cross_references":{"pubmed":["40697759"],"doi":["10.1016/j.fsisyn.2025.100623"]}}