biostudies-literatureUnknown10(7)Wu SThe colonization and development of the gut microbiota during early life, especially <i>Bifidobacterium</i>, may be influenced by maternal bacterial communities, including those of human milk. However, the interaction of bacteria in mother-infant dyads during breastfeeding remains unclear. This study focused primarily on the characteristics and dynamics of the infant gut and human milk microbiota within the first month of life on the basis of a birth cohort and explored the interaction of the microbiota derived from the two niches by sequencing and culture-based methods, especially <i>Bifidobacterium,</i> as the representative dominator in the infant gut. Infant feces and human milk samples from 21 mother-infant dyads were collected on days 0, 7, and 30 postpartum. The bacterial compositiomSystemse0048025https://www.ebi.ac.uk/biostudies/studies/S-EPMC12282193biostudies-literatureEarly life bifidobacterial mother-infant transmission: greater contribution from the infant gut to human milk revealed by microbiomic and culture-based methods.PMC12282193He FSu SLi JHuang TJiang FZhang XCheng RWu SLuo GHan WJia WMao YfalseEarly life bifidobacterial mother-infant transmission: greater contribution from the infant gut to human milk revealed by microbiomic and culture-based methods.The colonization and development of the gut microbiota during early life, especially <i>Bifidobacterium</i>, may be influenced by maternal bacterial communities, including those of human milk. However, the interaction of bacteria in mother-infant dyads during breastfeeding remains unclear. This study focused primarily on the characteristics and dynamics of the infant gut and human milk microbiota within the first month of life on the basis of a birth cohort and explored the interaction of the microbiota derived from the two niches by sequencing and culture-based methods, especially <i>Bifidobacterium,</i> as the representative dominator in the infant gut. Infant feces and human milk samples from 21 mother-infant dyads were collected on days 0, 7, and 30 postpartum. The bacterial compositio2025-01-01T00:00:00Z2025 Jul2026-03-27T15:26:00.998Z2025-08-23T03:09:25.195ZS-EPMC122821934055804610.1128/msystems.00480-25