{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen K"],"funding":["Key Realm R&D Program of Guangdong Province","National Natural Science Foundation of China","Guangdong Science and Technology Department","Key Realm R&D Program of Guangdong Province","Shenzhen Bay Scholars Program"],"pagination":["gkaf702"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12282948"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(14)"],"pubmed_abstract":["Purine riboswitches, located in the 5'-untranslated regions of bacterial messenger RNA, regulate gene expression by sensing purines and their derivatives. A class of the guanine-I riboswitch variants was recently reported to be the third class of 2'-deoxyguanosine (2'-dG) riboswitch termed 2'-dG-III riboswitch. Here we present the crystal structures of the 2'-dG-III riboswitch bound with 2'-dG, guanosine, or guanine. Despite similarities in secondary and overall structures to other purine riboswitches, the 2'-dG-III riboswitch exhibits unique features in its loop-loop interaction, three-way junction, and ligand binding. The 2'-dG-III riboswitch exhibits a tuning fork-like structure with a unique six-tiered interaction within the three-way junction. The second, third, and fourth tiers form the ligand-binding pocket, with a consistent binding mode for the guanine moiety of all three ligands. Structural and biochemical analyses reveal detailed interactions between the 2'-dG-III riboswitch and different ligands, providing insights into its regulatory mechanisms in purine metabolism."],"journal":["Nucleic acids research"],"pubmed_title":["Crystal structures reveal the distinct features of the 2'-dG-III riboswitch in the purine riboswitch family."],"pmcid":["PMC12282948"],"funding_grant_id":["32400021","32171191","2024A1515012594","2020B1212030004","2023B1212060013","2022B0202050003","32001639"],"pubmed_authors":["Peng X","Chen K","Liao W","Ren Y","Lu Z","Huang L","Wang J"],"additional_accession":[]},"is_claimable":false,"name":"Crystal structures reveal the distinct features of the 2'-dG-III riboswitch in the purine riboswitch family.","description":"Purine riboswitches, located in the 5'-untranslated regions of bacterial messenger RNA, regulate gene expression by sensing purines and their derivatives. A class of the guanine-I riboswitch variants was recently reported to be the third class of 2'-deoxyguanosine (2'-dG) riboswitch termed 2'-dG-III riboswitch. Here we present the crystal structures of the 2'-dG-III riboswitch bound with 2'-dG, guanosine, or guanine. Despite similarities in secondary and overall structures to other purine riboswitches, the 2'-dG-III riboswitch exhibits unique features in its loop-loop interaction, three-way junction, and ligand binding. The 2'-dG-III riboswitch exhibits a tuning fork-like structure with a unique six-tiered interaction within the three-way junction. The second, third, and fourth tiers form the ligand-binding pocket, with a consistent binding mode for the guanine moiety of all three ligands. Structural and biochemical analyses reveal detailed interactions between the 2'-dG-III riboswitch and different ligands, providing insights into its regulatory mechanisms in purine metabolism.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-07-01T03:15:36.16Z","creation":"2025-08-12T03:05:02.562Z"},"accession":"S-EPMC12282948","cross_references":{"pubmed":["40694847"],"doi":["10.1093/nar/gkaf702"]}}