biostudies-literatureUnknown15Wang Y<h4>Introduction</h4>Recent studies have underscored the role of interactions between Traditional Chinese Medicine (TCM) and the gut microbiome (GM) in mediating therapeutic effects. Jian-Pi-Yi-Shen Formula (JPYSF) has shown efficacy in ameliorating chronic kidney disease (CKD) symptoms, but its mechanisms via GM modulation remain unclear.<h4>Methods</h4>In this study, 8-week-old rats were assigned to three groups after a two-week acclimation: C (normal diet for six weeks), M (adenine diet for four weeks then normal diet for two weeks), and T (same as M, with JPYSF administered during the final three weeks). Fecal samples were collected at three timepoints (T1: post-acclimation; T2: after three weeks on respective diets; T3: after three weeks of JPYSF treatment) for metagenomic sequencing.Frontiers in cellular and infection microbiology1526863https://www.ebi.ac.uk/biostudies/studies/S-EPMC12283701biostudies-literatureJian-Pi-Yi-Shen formula improves kidney function by regulating gut microbiome in rats with chronic kidney disease.PMC12283701Lu JDai WWang YYang SfalseJian-Pi-Yi-Shen formula improves kidney function by regulating gut microbiome in rats with chronic kidney disease.<h4>Introduction</h4>Recent studies have underscored the role of interactions between Traditional Chinese Medicine (TCM) and the gut microbiome (GM) in mediating therapeutic effects. Jian-Pi-Yi-Shen Formula (JPYSF) has shown efficacy in ameliorating chronic kidney disease (CKD) symptoms, but its mechanisms via GM modulation remain unclear.<h4>Methods</h4>In this study, 8-week-old rats were assigned to three groups after a two-week acclimation: C (normal diet for six weeks), M (adenine diet for four weeks then normal diet for two weeks), and T (same as M, with JPYSF administered during the final three weeks). Fecal samples were collected at three timepoints (T1: post-acclimation; T2: after three weeks on respective diets; T3: after three weeks of JPYSF treatment) for metagenomic sequencing.2025-01-01T00:00:00Z20252026-03-16T04:21:20.129Z2025-08-14T03:05:53.086ZS-EPMC122837014070367110.3389/fcimb.2025.1526863