biostudies-literatureFarizano JVConsejo Nacional de Investigaciones Científicas y TécnicasAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación1624743https://www.ebi.ac.uk/biostudies/studies/S-EPMC12286952biostudies-literatureUnknown16Indwelling devices, such as double-J stents, are commonly used in urological surgery and are often associated with a high risk of urinary tract infections (UTIs) due to biofilm-related complications. In this study, we characterized 27 clinical bacterial isolates from double-J catheters, including <i>Staphylococcus</i> spp., <i>Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli</i>, and <i>Bacillus</i> spp., to investigate their pathogenic potential. Our findings revealed that strong biofilm producers (<i>E. coli, S. aureus</i>, and <i>B. subtilis</i>) exhibited robust extracellular matrix synthesis. Notably, multidrug resistance was observed in <i>E. coli, K. pneumoniae</i>, and <i>E. faecalis</i>. Mixed-culture experiments demonstrated that <i>Bacillus</i> spp. enhanced the biFrontiers in microbiologyVirulence traits and bacterial interactions within the complex microbial population in urinary double-J catheters.PMC12286952PIP11220200103262COPICT-2021-CAT-I-00159PICT 02977PICT 2021-GRF-TI-00306Castagnaro EArroyo-Egea JTFarizano JVVallejos ACSaavedra LGrillo-Puertas MAparicio JDRapisarda VAVillegas JMHebert EMfalseVirulence traits and bacterial interactions within the complex microbial population in urinary double-J catheters.Indwelling devices, such as double-J stents, are commonly used in urological surgery and are often associated with a high risk of urinary tract infections (UTIs) due to biofilm-related complications. In this study, we characterized 27 clinical bacterial isolates from double-J catheters, including <i>Staphylococcus</i> spp., <i>Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli</i>, and <i>Bacillus</i> spp., to investigate their pathogenic potential. Our findings revealed that strong biofilm producers (<i>E. coli, S. aureus</i>, and <i>B. subtilis</i>) exhibited robust extracellular matrix synthesis. Notably, multidrug resistance was observed in <i>E. coli, K. pneumoniae</i>, and <i>E. faecalis</i>. Mixed-culture experiments demonstrated that <i>Bacillus</i> spp. enhanced the bi2025-01-01T00:00:00Z20252026-05-29T05:02:36.964Z2025-08-17T03:06:04.582ZS-EPMC122869524070891710.3389/fmicb.2025.1624743