biostudies-literatureUnknown30(3)Zeng LROsteosarcoma (OS) is the most frequent type of cancer, as well as a common malignant tumor in orthopedics. Multiple long non-coding RNAs (lncRNAs) are implicated in immune infiltration in numerous types of cancer. Using single-sample gene set enrichment analysis to assign patients with OS, two immunotypes of OS were identified. Using K-means, spectral and PCA-K-means clustering two immunotypes of OS were demonstrated to be reproducible and represent a biologically meaningful classification of patients with OS based on their tumor immune microenvironment. Cluster 1 was an immune-infiltrating type, while Cluster 2 was an immune 'desert' type (low immune cell infiltration). Between Cluster 1 and 2, 29 common differentially expressed lncRNAs (DELs) were identified and univariate Cox regressionOncology letters443https://www.ebi.ac.uk/biostudies/studies/S-EPMC12288445biostudies-literatureRisk score model with two immune infiltration-related long non-coding RNAs to predict prognosis in patients with osteosarcoma.PMC12288445Yang GZeng LRZhu GHMei HBfalseRisk score model with two immune infiltration-related long non-coding RNAs to predict prognosis in patients with osteosarcoma.Osteosarcoma (OS) is the most frequent type of cancer, as well as a common malignant tumor in orthopedics. Multiple long non-coding RNAs (lncRNAs) are implicated in immune infiltration in numerous types of cancer. Using single-sample gene set enrichment analysis to assign patients with OS, two immunotypes of OS were identified. Using K-means, spectral and PCA-K-means clustering two immunotypes of OS were demonstrated to be reproducible and represent a biologically meaningful classification of patients with OS based on their tumor immune microenvironment. Cluster 1 was an immune-infiltrating type, while Cluster 2 was an immune 'desert' type (low immune cell infiltration). Between Cluster 1 and 2, 29 common differentially expressed lncRNAs (DELs) were identified and univariate Cox regression2025-01-01T00:00:00Z2025 Sep2026-03-31T10:49:13.558Z2025-08-28T03:05:56.302ZS-EPMC122884454070859610.3892/ol.2025.15189