biostudies-literatureD'Amelia VTRacking down the balance between polyphenols ANd Senescence for biotic stress tolerance In potaTo878-889https://www.ebi.ac.uk/biostudies/studies/S-EPMC12290281biostudies-literatureUnknown66(6)Events of duplication and neo/subfunctionalization have significantly expanded the functional roles of R2R3 myeloblastosis (MYB) transcription factors in plants. In a previous study, we demonstrated that two paralogous R2R3 MYBs from Solanum tuberosum and S. commersonii, AN1 and AN2, respectively, induce anthocyanin pigmentation to varying extents when transiently overexpressed. However, questions related to the distinct functions of these genes remained unanswered. In this study, we further investigated these genes by comparing transgenic tobacco plants that constitutively overexpress AN1 and AN2. We observed differences between AN1 and AN2 that not only influenced plant pigmentation but also impacted the structural features of vascular tissues. Both genes promoted the accumulation of phePlant & cell physiologyExpanded functional roles of R2R3-MYB (S6) transcription factors in balancing phenylpropanoid and phenolamide pathways in Solanaceae.PMC12290281Grant Assignment Decree No. D. D. n. 1048 adoptedCirillo VChiaiese PPiccinelli ALD'Orso FAversano RStaiti ADocimo TD'Amelia VPossenti MMaggio ACarputo DfalseExpanded functional roles of R2R3-MYB (S6) transcription factors in balancing phenylpropanoid and phenolamide pathways in Solanaceae.Events of duplication and neo/subfunctionalization have significantly expanded the functional roles of R2R3 myeloblastosis (MYB) transcription factors in plants. In a previous study, we demonstrated that two paralogous R2R3 MYBs from Solanum tuberosum and S. commersonii, AN1 and AN2, respectively, induce anthocyanin pigmentation to varying extents when transiently overexpressed. However, questions related to the distinct functions of these genes remained unanswered. In this study, we further investigated these genes by comparing transgenic tobacco plants that constitutively overexpress AN1 and AN2. We observed differences between AN1 and AN2 that not only influenced plant pigmentation but also impacted the structural features of vascular tissues. Both genes promoted the accumulation of phe2025-01-01T00:00:00Z2025 Jul2026-04-08T00:39:54.81Z2025-08-13T03:04:38.971ZS-EPMC122902814009828010.1093/pcp/pcaf028