biostudies-literaturePires Costa EConselho Nacional de Desenvolvimento Cient?fico e Tecnol?gicoFunda??o de Amparo ? Pesquisa do Estado de Minas GeraisCoordena??o de Aperfei?oamento de Pessoal de N?vel Superior31033-31045https://www.ebi.ac.uk/biostudies/studies/S-EPMC12290672biostudies-literatureUnknown10(28)Different therapeutic approaches, particularly those involving plant-derived compounds, have been explored for treating inflammatory diseases. This study evaluated the antioxidant and anti-inflammatory properties of eugenol (EU) in its pure form, bis-eugenol (BIS), and clove essential oil (OE) at different concentrations (5, 10, and 25 μg/mL). Chemical analysis confirmed that OE contains a high proportion of eugenol (45-90%), with the presence of eugenol acetate differentiating it from pure eugenol, while bis-eugenol was successfully synthesized and characterized with a 97% yield. Antioxidant activity assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed that 25 μg/mL of each compound effectively neutralized free radicals and reduced feACS omegaAntioxidant and Anti-inflammatory Activity of Eugenol, Bis-eugenol, and Clove Essential Oil: An In Vitro Study.PMC12290672001310413/2023-0403194/2023-7APQ-03519-22306733/2023-4APQ-04164-22Maciel Dos Santos Mda Silva DATeixeira RRde Paula RAPires Costa ELopes RPGoncalves RVfalseAntioxidant and Anti-inflammatory Activity of Eugenol, Bis-eugenol, and Clove Essential Oil: An In Vitro Study.Different therapeutic approaches, particularly those involving plant-derived compounds, have been explored for treating inflammatory diseases. This study evaluated the antioxidant and anti-inflammatory properties of eugenol (EU) in its pure form, bis-eugenol (BIS), and clove essential oil (OE) at different concentrations (5, 10, and 25 μg/mL). Chemical analysis confirmed that OE contains a high proportion of eugenol (45-90%), with the presence of eugenol acetate differentiating it from pure eugenol, while bis-eugenol was successfully synthesized and characterized with a 97% yield. Antioxidant activity assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed that 25 μg/mL of each compound effectively neutralized free radicals and reduced fe2025-01-01T00:00:00Z2025 Jul2026-05-09T19:10:20.413Z2026-04-08T01:10:42.61ZS-EPMC122906724072782810.1021/acsomega.5c04146