biostudies-literatureUnknown10(28)Zheng MThe detection of is crucial for the diagnosis and treatment of infectious diseases. However, developing a simple and sensitive method for detection remains challenging. Herein, we propose a sensitive surface-enhanced Raman spectroscopy (SERS) platform for the detection of using core-shell aptamer-Au@Fe₃O₄ nanoparticles (Fe₃O₄-Au@apt) and freezing SERS tags. Core-shell Au@Fe₃O₄ magnetic nanoparticles were modified with specific primary aptamers for precise and efficient capture of , followed by modification with large amounts of secondary aptamers and tetramercaptobenzoic acid through freezing. The proposed SERS platform demonstrated significant sensitivity toward , with a limit of detection of 6.91 CFU/mL, which demonstrates 99.25 times higher sensitivity than conventional SERS tags. Additionally, the proposed SERS platform achieved good recovery rates in human urine and serum samples, ranging from 97.7% to 104.0% and 90.5% to 104.7%. The findings of the study suggest that the proposed SERS platform holds promise for the rapid and efficient detection of pathogenic bacteria.ACS omega30651-30659https://www.ebi.ac.uk/biostudies/studies/S-EPMC12290702biostudies-literatureSensitive SERS Platform for the Detection of via Freezing SERS Tags and Core-Shell Aptamer-Au@Fe<sub>3</sub>O<sub>4</sub> Nanoparticles.PMC12290702Zhang WSun LWang QZhu JZheng MXiao YChen LLv SLin HLuo SfalseSensitive SERS Platform for the Detection of via Freezing SERS Tags and Core-Shell Aptamer-Au@Fe<sub>3</sub>O<sub>4</sub> Nanoparticles.The detection of is crucial for the diagnosis and treatment of infectious diseases. However, developing a simple and sensitive method for detection remains challenging. Herein, we propose a sensitive surface-enhanced Raman spectroscopy (SERS) platform for the detection of using core-shell aptamer-Au@Fe₃O₄ nanoparticles (Fe₃O₄-Au@apt) and freezing SERS tags. Core-shell Au@Fe₃O₄ magnetic nanoparticles were modified with specific primary aptamers for precise and efficient capture of , followed by modification with large amounts of secondary aptamers and tetramercaptobenzoic acid through freezing. The proposed SERS platform demonstrated significant sensitivity toward , with a limit of detection of 6.91 CFU/mL, which demonstrates 99.25 times higher sensitivity than conventional SERS tags. Additionally, the proposed SERS platform achieved good recovery rates in human urine and serum samples, ranging from 97.7% to 104.0% and 90.5% to 104.7%. The findings of the study suggest that the proposed SERS platform holds promise for the rapid and efficient detection of pathogenic bacteria.2025-01-01T00:00:00Z2025 Jul2026-05-09T17:54:22.919Z2026-04-08T01:08:22.322ZS-EPMC122907024072774710.1021/acsomega.5c02638