biostudies-literatureLeonteva AState program of the «Sirius» Federal Territory «Scientific and technological development of the «Sirius» Federal Territory»1039https://www.ebi.ac.uk/biostudies/studies/S-EPMC12293796biostudies-literatureUnknown14(14)Due to the complexity of modeling tumor-host interactions within the tumor microenvironment in vitro, we developed a 3D heterotypic cellular breast cancer (BC) model. We generated spheroid models using MCF7, MDA-MB-231, and SK-BR-3 cell lines alongside cancer-associated (BrC4f) and normal (BN120f) fibroblasts in ultra-low attachment plates. Stromal spheroids (3Df) were formed using a liquid overlay technique (graphical abstract). The YT cell line and peripheral blood NK (PB-NK) cells were used as immune components in our 3D model. In this study, we showed that stromal cells promoted tumor cell aggregation into spheroids, regardless of the initial proliferation rates, with NK cells accumulating in fibroblast-rich regions. The presence of CAFs within the model induced alterations in the exprCellsThe Activity of Human NK Cells Towards 3D Heterotypic Cellular Tumor Model of Breast Cancer.PMC12293796Agreement №27-03 date 27.09.2024Leonteva ARichter VYurina ATroitskaya OBogachek MKuligina EBelovezhets TNushtaeva AAbdurakhmanova MKulemzin SfalseThe Activity of Human NK Cells Towards 3D Heterotypic Cellular Tumor Model of Breast Cancer.Due to the complexity of modeling tumor-host interactions within the tumor microenvironment in vitro, we developed a 3D heterotypic cellular breast cancer (BC) model. We generated spheroid models using MCF7, MDA-MB-231, and SK-BR-3 cell lines alongside cancer-associated (BrC4f) and normal (BN120f) fibroblasts in ultra-low attachment plates. Stromal spheroids (3Df) were formed using a liquid overlay technique (graphical abstract). The YT cell line and peripheral blood NK (PB-NK) cells were used as immune components in our 3D model. In this study, we showed that stromal cells promoted tumor cell aggregation into spheroids, regardless of the initial proliferation rates, with NK cells accumulating in fibroblast-rich regions. The presence of CAFs within the model induced alterations in the expr2025-01-01T00:00:00Z2025 Jul2025-08-13T03:04:30.378Z2025-08-13T03:04:30.378ZS-EPMC122937964071029210.3390/cells14141039