{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shah KM"],"funding":["Biotechnology and Biological Sciences Research Council"],"pagination":["1-24"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12296108"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(1)"],"pubmed_abstract":["MicroRNA-mediated gene silencing is a conserved mechanism of post-transcriptional gene regulation across metazoans. It depends on base pairing between small RNAs and mRNAs, and on protein complexes including the RNA-induced silencing complex (RISC), where Argonaute 2 (AGO2) plays a central role. A full understanding of RNA silencing requires reliable molecular tools to study AGO2 and RISC. Affinity tagging and antibody-based methods can introduce artefacts, and both the <i>N</i>- and C-terminal domains of AGO2 are critical for its function. While N-terminal tags are frequently used, and a recent study in mice showed altered activity in N-terminal HaloTag-AGO2 fusions, the consequences of C-terminal tagging remain underexplored. CRISPaint, a CRISPR-Cas9-based technique, enables endogenous C"],"journal":["RNA biology"],"pubmed_title":["C-terminal tagging impairs AGO2 function."],"pmcid":["PMC12296108"],"funding_grant_id":["BB/V009567/1"],"pubmed_authors":["Sharp TV","Shah KM","Crozier AFF","Assaraf A","Plevin MJ","Mardakheh F","Grevitt P","Arya M"],"additional_accession":[]},"is_claimable":false,"name":"C-terminal tagging impairs AGO2 function.","description":"MicroRNA-mediated gene silencing is a conserved mechanism of post-transcriptional gene regulation across metazoans. It depends on base pairing between small RNAs and mRNAs, and on protein complexes including the RNA-induced silencing complex (RISC), where Argonaute 2 (AGO2) plays a central role. A full understanding of RNA silencing requires reliable molecular tools to study AGO2 and RISC. Affinity tagging and antibody-based methods can introduce artefacts, and both the <i>N</i>- and C-terminal domains of AGO2 are critical for its function. While N-terminal tags are frequently used, and a recent study in mice showed altered activity in N-terminal HaloTag-AGO2 fusions, the consequences of C-terminal tagging remain underexplored. CRISPaint, a CRISPR-Cas9-based technique, enables endogenous C","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-03-15T18:40:32.596Z","creation":"2025-08-13T03:04:36.945Z"},"accession":"S-EPMC12296108","cross_references":{"pubmed":["40698645"],"doi":["10.1080/15476286.2025.2534028"]}}