{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(7)"],"submitter":["Wang L"],"funding":["Westlake University"],"pubmed_abstract":["RNA structure plays a crucial role in its function and undergoes dynamic changes throughout its life cycle. To study these dynamics, we developed TAS sequencing (TAS-seq), which expresses the deaminase TadA-8e in specific subcellular compartments to modify single-stranded adenosines, particularly within hairpin loops. We applied TAS-seq to the nucleus, cytosol, and endoplasmic reticulum membrane, identifying adenosine structural variations and compartment-specific regulation of RNA stability. Single-cell TAS-seq revealed structural heterogeneity of cytosolic RNAs. Additionally, adenosines labeled by TAS-seq contribute to guide RNA optimization in the CRISPR-Cas13d system. Our method provides insights into compartment-specific RNA structural dynamics, cell-specific heterogeneity, and their "],"journal":["Cell reports methods"],"pagination":["101087"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12296453"],"repository":["biostudies-literature"],"pubmed_title":["TAS-seq enables subcellular single-stranded adenosine profiling by signal peptide-assisted adenosine deamination."],"pmcid":["PMC12296453"],"pubmed_authors":["Ma L","Wu P","Lu Z","Zhou Y","Yu Z","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"TAS-seq enables subcellular single-stranded adenosine profiling by signal peptide-assisted adenosine deamination.","description":"RNA structure plays a crucial role in its function and undergoes dynamic changes throughout its life cycle. To study these dynamics, we developed TAS sequencing (TAS-seq), which expresses the deaminase TadA-8e in specific subcellular compartments to modify single-stranded adenosines, particularly within hairpin loops. We applied TAS-seq to the nucleus, cytosol, and endoplasmic reticulum membrane, identifying adenosine structural variations and compartment-specific regulation of RNA stability. Single-cell TAS-seq revealed structural heterogeneity of cytosolic RNAs. Additionally, adenosines labeled by TAS-seq contribute to guide RNA optimization in the CRISPR-Cas13d system. Our method provides insights into compartment-specific RNA structural dynamics, cell-specific heterogeneity, and their ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-07-15T05:59:56.341Z","creation":"2025-08-13T03:04:14.159Z"},"accession":"S-EPMC12296453","cross_references":{"pubmed":["40570838"],"doi":["10.1016/j.crmeth.2025.101087"]}}