{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li E"],"funding":["National Eye Research Centre","U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH)","NIA NIH HHS","U.S. Department of Health &amp; Human Services | NIH | National Institute on Aging","NIMH NIH HHS","National Eye Research Centre (NERC)","U.S. Department of Health & Human Services | NIH | National Institute on Aging (U.S. National Institute on Aging)","U.S. Department of Health &amp; Human Services | NIH | National Institute of Mental Health"],"pagination":["6902"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12297475"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["During mouse embryonic brain development, the Evf2 ultraconserved enhancer (UCE) lncRNA guides the Dlx5/6UCE to ~129 sites across chr6. However, previous work identified only 4 transcriptionally regulated targets associated with Evf2-Dlx5/6UCE-gene guidance, raising questions about the significance of Evf2-regulated Dlx5/6UCE-gene interactions. Here, single-cell transcriptomics reveal far greater alignment between Evf2-Dlx5/6UCE-gene guidance and transcriptional regulation than previously reported. Evf2 divides chr6 into short-range ( <10 Mb distant), activated genes, and long/super-long-range (10-129 Mb distant), repressed genes, identifying seizure regulating genes in the embryonic subventricular zone that predict adult phenotypes. Evf2-regulated Dlx5/6UCE-gene distances and directions ("],"journal":["Nature communications"],"pubmed_title":["Single-cell transcriptomics of ventral forebrain progenitors identifies Evf2 enhancer lncRNA-enhancer gene guidance through direct RNA binding and RNP recruitment domains."],"pmcid":["PMC12297475"],"funding_grant_id":["R01MH111267","R03MH126145","R03 MH126145","P30EY002687","RF1 AG068140","RF1AG068140","R01 MH111267"],"pubmed_authors":["Cajigas I","Ay F","Kohtz SJ","Hinojosa-Gonzalez L","Li E","Vassar RJ","Chakraborty A","Shiau F","Bertossi R","Clark BS","Kessler JA","Kohtz JD"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptomics of ventral forebrain progenitors identifies Evf2 enhancer lncRNA-enhancer gene guidance through direct RNA binding and RNP recruitment domains.","description":"During mouse embryonic brain development, the Evf2 ultraconserved enhancer (UCE) lncRNA guides the Dlx5/6UCE to ~129 sites across chr6. However, previous work identified only 4 transcriptionally regulated targets associated with Evf2-Dlx5/6UCE-gene guidance, raising questions about the significance of Evf2-regulated Dlx5/6UCE-gene interactions. Here, single-cell transcriptomics reveal far greater alignment between Evf2-Dlx5/6UCE-gene guidance and transcriptional regulation than previously reported. Evf2 divides chr6 into short-range ( <10 Mb distant), activated genes, and long/super-long-range (10-129 Mb distant), repressed genes, identifying seizure regulating genes in the embryonic subventricular zone that predict adult phenotypes. Evf2-regulated Dlx5/6UCE-gene distances and directions (","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-07-15T09:44:01.878Z","creation":"2025-08-28T03:09:18.489Z"},"accession":"S-EPMC12297475","cross_references":{"pubmed":["40715144"],"doi":["10.1038/s41467-025-62205-y"]}}