{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ye N"],"funding":["The Natural Science Foundation Project of the Science; The Science and Technology Department of Jilin Province and the Jilin Provincial Key Research and Development Program"],"pagination":["2218"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12297902"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(14)"],"pubmed_abstract":["In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt-alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i>GmPKS4</i> gene was experimentally shown to be involved in salt-alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the <i>GmSNF4</i> gene was induced by salt-alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of <i>GmSNF4</i>'s role in resistance to salt-alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, <i>GmSNF4</i> may play a significant role in plant salt-alkali stress tolerance."],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Soybean &lt;i&gt;GmSNF4&lt;/i&gt; Confers Salt-Alkali Stress Tolerance in Transgenic Plants."],"pmcid":["PMC12297902"],"funding_grant_id":["20250102324JC; 20240304155SF"],"pubmed_authors":["Wang N","Lv HY","Ye N","Dong YY","Bian JS","Zhou BH","Yong LT","Liu WC","Yang T","Li XW","Wang FW"],"additional_accession":[]},"is_claimable":false,"name":"Soybean &lt;i&gt;GmSNF4&lt;/i&gt; Confers Salt-Alkali Stress Tolerance in Transgenic Plants.","description":"In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, <i>GmSNF4</i>, which promotes plant tolerance to salt-alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The <i>GmPKS4</i> gene was experimentally shown to be involved in salt-alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the <i>GmSNF4</i> gene was induced by salt-alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of <i>GmSNF4</i>'s role in resistance to salt-alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, <i>GmSNF4</i> may play a significant role in plant salt-alkali stress tolerance.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2025-08-18T09:54:11.113Z","creation":"2025-08-18T09:54:11.113Z"},"accession":"S-EPMC12297902","cross_references":{"pubmed":["40733455"],"doi":["10.3390/plants14142218"]}}