biostudies-literatureNie Tkunpeng cuijiahui wangluwang1474https://www.ebi.ac.uk/biostudies/studies/S-EPMC12297972biostudies-literatureUnknown13(7)The strain of <i>Oceanobacillus picturae</i> DY09, as a typical halophilic microorganism, possesses distinctive salt adaptation mechanisms that hold significant application value in the fields of agriculture, industry, and biomedicine. To deeply analyze the salt-tolerance molecular mechanism of this strain, this research disclosed its salt-tolerance strategies under diverse salt concentrations through transcriptomics. In a low-salt environment, the DY09 strain adopted a "metabolic simplification" strategy, significantly reducing the metabolic load by promoting lysine degradation and inhibiting the biosynthesis of branched-chain amino acids and glycine betaine (GB) but upregulating the expression of the GB transporter gene <i>betH</i> and preferentially utilizing exogenous GB to maintain baMicroorganismsA Halophilic Bacterium for Bioremediation of Saline-Alkali Land: The Triadic and Synergetic Response Mechanism of &lt;i&gt;Oceanobacillus picturae&lt;/i&gt; DY09 to Salt Stress.PMC12297972ZR2024QD159ZR2023QB272ZR2022QC177Liu YNie TFu SCui KWang JWang LfalseA Halophilic Bacterium for Bioremediation of Saline-Alkali Land: The Triadic and Synergetic Response Mechanism of &lt;i&gt;Oceanobacillus picturae&lt;/i&gt; DY09 to Salt Stress.The strain of <i>Oceanobacillus picturae</i> DY09, as a typical halophilic microorganism, possesses distinctive salt adaptation mechanisms that hold significant application value in the fields of agriculture, industry, and biomedicine. To deeply analyze the salt-tolerance molecular mechanism of this strain, this research disclosed its salt-tolerance strategies under diverse salt concentrations through transcriptomics. In a low-salt environment, the DY09 strain adopted a "metabolic simplification" strategy, significantly reducing the metabolic load by promoting lysine degradation and inhibiting the biosynthesis of branched-chain amino acids and glycine betaine (GB) but upregulating the expression of the GB transporter gene <i>betH</i> and preferentially utilizing exogenous GB to maintain ba2025-01-01T00:00:00Z2025 Jun2025-08-14T03:05:49.351Z2025-08-14T03:05:49.351ZS-EPMC122979724073198410.3390/microorganisms13071474