biostudies-literatureXu YNatural Sciences and Engineering Research Council of CanadaAgriculture and Agrifoods Canada, AgSci BioCluster1463https://www.ebi.ac.uk/biostudies/studies/S-EPMC12299085biostudies-literatureUnknown13(7)Mannosyl erythritol lipids (MELs) are glycolipid biosurfactants produced by <i>Ustilaginomycete</i> yeasts. The MEL biosynthetic pathway has been characterized in <i>Ustilago maydis</i> where a putative transporter encoded by <i>MMF1</i> is required for the secretion of the glycolipid surfactant to the extracellular space. The anamorphic yeast <i>Moesziomyces antarcticus</i> is a prolific producer of MELs, but the mechanism of MEL secretion is less well characterized than in <i>U. maydis</i>. Homologous recombination was employed to generate a disruption of the <i>MMF1</i> gene in <i>M. antarcticus</i> JCM10317. This mutation did not prevent the intracellular accumulation of MEL species but did result in significantly reduced secretion of the conventional MEL-A, MEL-B and MEL-C species detMicroorganisms<i>Moesziomyces antarcticus MMF1</i> Has a Role in the Secretion of Mannosylerythritol Lipids.PMC12299085RGPIN-2021-0289853Stuart DTXu Yfalse<i>Moesziomyces antarcticus MMF1</i> Has a Role in the Secretion of Mannosylerythritol Lipids.Mannosyl erythritol lipids (MELs) are glycolipid biosurfactants produced by <i>Ustilaginomycete</i> yeasts. The MEL biosynthetic pathway has been characterized in <i>Ustilago maydis</i> where a putative transporter encoded by <i>MMF1</i> is required for the secretion of the glycolipid surfactant to the extracellular space. The anamorphic yeast <i>Moesziomyces antarcticus</i> is a prolific producer of MELs, but the mechanism of MEL secretion is less well characterized than in <i>U. maydis</i>. Homologous recombination was employed to generate a disruption of the <i>MMF1</i> gene in <i>M. antarcticus</i> JCM10317. This mutation did not prevent the intracellular accumulation of MEL species but did result in significantly reduced secretion of the conventional MEL-A, MEL-B and MEL-C species det2025-01-01T00:00:00Z2025 Jun2026-06-30T03:28:53.766Z2025-08-14T03:05:53.311ZS-EPMC122990854073197310.3390/microorganisms13071463