biostudies-literatureSampaio BAFundação de amparo a pesquisa do estado da bahia746https://www.ebi.ac.uk/biostudies/studies/S-EPMC12299757biostudies-literatureUnknown13(7)<b>Background/Objectives</b>: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to existing immunoprophylactic measures, this study aimed to develop a recombinant subunit vaccine against <i>M. agalactiae</i> and evaluate its specific immune response in goats. <b>Methods</b>: Goats were divided into three groups: group 1 received recombinant proteins (P40 and MAG_1560), group 2 received formalin-inactivated <i>M. agalactiae</i>, and group 3 received Tris-buffered saline (negative control). All solutions were emulsified in Freund's adjuvant. Animals were monitored for 181 days. IgG antibody prVaccinesImmune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats.PMC12299757APP0006/2023 and DCR0002/2023Bastos BLCampos GBMarques LMBarbosa MSAndres ESSGuimaraes BCBChopra-Dewasthaly RSampaio BAGomes CPBittencourt RSda Silva LSCda Cruz JFde Oliveira MGSantos Junior MNTimenetsky Jda Silva AMBCorreia TMLfalseImmune Responses Induced by Recombinant Membrane Proteins of <i>Mycoplasma agalactiae</i> in Goats.<b>Background/Objectives</b>: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to existing immunoprophylactic measures, this study aimed to develop a recombinant subunit vaccine against <i>M. agalactiae</i> and evaluate its specific immune response in goats. <b>Methods</b>: Goats were divided into three groups: group 1 received recombinant proteins (P40 and MAG_1560), group 2 received formalin-inactivated <i>M. agalactiae</i>, and group 3 received Tris-buffered saline (negative control). All solutions were emulsified in Freund's adjuvant. Animals were monitored for 181 days. IgG antibody pr2025-01-01T00:00:00Z2025 Jul2025-08-14T03:06:31.07Z2025-08-14T03:06:31.07ZS-EPMC122997574073372310.3390/vaccines13070746