{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li X"],"funding":["Jilin Provincial Science and Technology Project","Project for Enhancing the Independent Innovation Capacity of Major Grain Crops"],"pagination":["2207"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12299880"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(14)"],"pubmed_abstract":["Soybean is a dual-purpose crop for food and oil, playing a crucial role in China's grain production. Seed size and weight are key agronomic traits directly influencing the yield. Cytokinin oxidases/dehydrogenases (CKXs) specifically degrade certain isoforms of endogenous cytokinins (CKs), thereby modulating plant growth and seed development. However, their role in soybeans remains largely uncharacterized. In a previous genome-wide association study of 250 soybean core germplasms, we identified <i>GmCKX3</i> as a yield-related gene. To elucidate its function, we developed <i>GmCKX3</i>-deficient mutants using CRISPR/Cas9 gene editing in soybean Williams82 and conducted a three-year phenotypic analysis. Loss of <i>GmCKX3</i> function significantly enhanced the seed size and weight, which was"],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Knockout of &lt;i&gt;GmCKX3&lt;/i&gt; Enhances Soybean Seed Yield via Cytokinin-Mediated Cell Expansion and Lipid Accumulation."],"pmcid":["PMC12299880"],"funding_grant_id":["2024C002","20240303012NC, 20210202002NC"],"pubmed_authors":["Qian X","Li X","Chen Z","Zhang Y","Zhao F","Niu L","Han S","Hao D"],"additional_accession":[]},"is_claimable":false,"name":"Knockout of &lt;i&gt;GmCKX3&lt;/i&gt; Enhances Soybean Seed Yield via Cytokinin-Mediated Cell Expansion and Lipid Accumulation.","description":"Soybean is a dual-purpose crop for food and oil, playing a crucial role in China's grain production. Seed size and weight are key agronomic traits directly influencing the yield. Cytokinin oxidases/dehydrogenases (CKXs) specifically degrade certain isoforms of endogenous cytokinins (CKs), thereby modulating plant growth and seed development. However, their role in soybeans remains largely uncharacterized. In a previous genome-wide association study of 250 soybean core germplasms, we identified <i>GmCKX3</i> as a yield-related gene. To elucidate its function, we developed <i>GmCKX3</i>-deficient mutants using CRISPR/Cas9 gene editing in soybean Williams82 and conducted a three-year phenotypic analysis. Loss of <i>GmCKX3</i> function significantly enhanced the seed size and weight, which was","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-06-30T03:27:28.819Z","creation":"2025-08-16T03:07:03.436Z"},"accession":"S-EPMC12299880","cross_references":{"pubmed":["40733444"],"doi":["10.3390/plants14142207"]}}