biostudies-literatureLi XJilin Provincial Science and Technology ProjectProject for Enhancing the Independent Innovation Capacity of Major Grain Crops2207https://www.ebi.ac.uk/biostudies/studies/S-EPMC12299880biostudies-literatureUnknown14(14)Soybean is a dual-purpose crop for food and oil, playing a crucial role in China's grain production. Seed size and weight are key agronomic traits directly influencing the yield. Cytokinin oxidases/dehydrogenases (CKXs) specifically degrade certain isoforms of endogenous cytokinins (CKs), thereby modulating plant growth and seed development. However, their role in soybeans remains largely uncharacterized. In a previous genome-wide association study of 250 soybean core germplasms, we identified <i>GmCKX3</i> as a yield-related gene. To elucidate its function, we developed <i>GmCKX3</i>-deficient mutants using CRISPR/Cas9 gene editing in soybean Williams82 and conducted a three-year phenotypic analysis. Loss of <i>GmCKX3</i> function significantly enhanced the seed size and weight, which wasPlants (Basel, Switzerland)Knockout of &lt;i&gt;GmCKX3&lt;/i&gt; Enhances Soybean Seed Yield via Cytokinin-Mediated Cell Expansion and Lipid Accumulation.PMC122998802024C00220240303012NC, 20210202002NCQian XLi XChen ZZhang YZhao FNiu LHan SHao DfalseKnockout of &lt;i&gt;GmCKX3&lt;/i&gt; Enhances Soybean Seed Yield via Cytokinin-Mediated Cell Expansion and Lipid Accumulation.Soybean is a dual-purpose crop for food and oil, playing a crucial role in China's grain production. Seed size and weight are key agronomic traits directly influencing the yield. Cytokinin oxidases/dehydrogenases (CKXs) specifically degrade certain isoforms of endogenous cytokinins (CKs), thereby modulating plant growth and seed development. However, their role in soybeans remains largely uncharacterized. In a previous genome-wide association study of 250 soybean core germplasms, we identified <i>GmCKX3</i> as a yield-related gene. To elucidate its function, we developed <i>GmCKX3</i>-deficient mutants using CRISPR/Cas9 gene editing in soybean Williams82 and conducted a three-year phenotypic analysis. Loss of <i>GmCKX3</i> function significantly enhanced the seed size and weight, which was2025-01-01T00:00:00Z2025 Jul2026-06-30T03:27:28.819Z2025-08-16T03:07:03.436ZS-EPMC122998804073344410.3390/plants14142207