biostudies-literatureFerreira CSUniversité de Strasbourg the Agence Nationale de la Recherche 'Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPESFundação de Amparo à Pesquisa de Minas Gerais-FAPEMIGConselho Nacional de Desenvolvimento Científico e Tecnológico-CNPqLabEx Chemistry of Complex SystemsFoundation Jean-Marie Lehn/Interdisciplinary Thematic Institute SysChem/IdExUnistra952https://www.ebi.ac.uk/biostudies/studies/S-EPMC12299903biostudies-literatureUnknown18(7)<b>Background/Objectives:</b> The increasing prevalence of multidrug-resistant bacteria presents a major global health challenge, prompting a search for innovative antimicrobial strategies. This study aimed to develop and evaluate a novel nanobiostructure combining alumina nanoparticles (NPs) with the antimicrobial peptide lunatin-1 (Lun-1), forming peptide-functionalized nanofilaments. The main objective was to investigate how the site of peptide functionalization (C-terminal vs. N-terminal) affects membrane interactions and antibacterial activity. <b>Methods</b>: NP-peptide conjugates were synthesized via covalent bonding between lun-1 and alumina NP and characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), zeta potential analysis, dynamic light scattering Pharmaceuticals (Basel, Switzerland)Influence of Peptide Conjugation Sites on Lunatin-Alumina Nanoparticles: Implications for Membrane Interaction and Antimicrobial Activity.PMC12299903CAPES- COFECUB program 88881.879071/2023-01ANR-10-IDEX-000210-LABX-0026_CSC438054/2018-0APQ-02238-17RED-00185-23projects Naturalarsenal 19-AMRB-0004-02, AmphiPep 20-CE18-0021, and SAFEST 21-CE18-0043)Nunes LOSalnikov ESBechinger BVerly RMKato KCFerreira CSCosta LMFde Souza KRde Castro Pimenta AMAraujo GPResende JMMartins HRfalseInfluence of Peptide Conjugation Sites on Lunatin-Alumina Nanoparticles: Implications for Membrane Interaction and Antimicrobial Activity.<b>Background/Objectives:</b> The increasing prevalence of multidrug-resistant bacteria presents a major global health challenge, prompting a search for innovative antimicrobial strategies. This study aimed to develop and evaluate a novel nanobiostructure combining alumina nanoparticles (NPs) with the antimicrobial peptide lunatin-1 (Lun-1), forming peptide-functionalized nanofilaments. The main objective was to investigate how the site of peptide functionalization (C-terminal vs. N-terminal) affects membrane interactions and antibacterial activity. <b>Methods</b>: NP-peptide conjugates were synthesized via covalent bonding between lun-1 and alumina NP and characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), zeta potential analysis, dynamic light scattering 2025-01-01T00:00:00Z2025 Jun2025-08-13T03:04:44.249Z2025-08-13T03:04:44.249ZS-EPMC122999034073224310.3390/ph18070952