biostudies-literatureUnknown10(7)Saucedo-Montalvo MC<i>Triatoma dimidiata</i> is a widely distributed vector of <i>Trypanosoma cruzi</i> in Mexico and Central America, found across a range of habitats from sylvatic to domestic. Vector control has relied heavily on indoor residual spraying with pyrethroids; however, reinfestation and emerging resistance have limited its long-term effectiveness. In this study, we analyzed the genetic diversity and population structure of <i>T. dimidiata</i> from Veracruz, Oaxaca, and Yucatan using mitochondrial markers (cyt b and ND4) and screened for knockdown resistance (kdr)-type mutations in the voltage-gated sodium channel (VGSC) gene. High haplotype diversity and regional differentiation were observed, with most genetic variation occurring between populations. The ND4 marker provided greater resolution Tropical medicine and infectious disease182https://www.ebi.ac.uk/biostudies/studies/S-EPMC12299954biostudies-literatureFirst Report of the L925I kdr Mutation Associated with Pyrethroid Resistance in Genetically Distinct <i>Triatoma dimidiata</i>, Vector of Chagas Disease in Mexico.PMC12299954Gutierrez-Rodriguez SMReyes-Solis GDCLopez-Monroy BDavila-Barboza JAFavela-Lara SFlores AESaucedo-Montalvo MCBobadilla-Utrera CRodriguez-Sanchez IPfalseFirst Report of the L925I kdr Mutation Associated with Pyrethroid Resistance in Genetically Distinct <i>Triatoma dimidiata</i>, Vector of Chagas Disease in Mexico.<i>Triatoma dimidiata</i> is a widely distributed vector of <i>Trypanosoma cruzi</i> in Mexico and Central America, found across a range of habitats from sylvatic to domestic. Vector control has relied heavily on indoor residual spraying with pyrethroids; however, reinfestation and emerging resistance have limited its long-term effectiveness. In this study, we analyzed the genetic diversity and population structure of <i>T. dimidiata</i> from Veracruz, Oaxaca, and Yucatan using mitochondrial markers (cyt b and ND4) and screened for knockdown resistance (kdr)-type mutations in the voltage-gated sodium channel (VGSC) gene. High haplotype diversity and regional differentiation were observed, with most genetic variation occurring between populations. The ND4 marker provided greater resolution 2025-01-01T00:00:00Z2025 Jun2025-08-17T03:05:59.259Z2025-08-17T03:05:59.259ZS-EPMC122999544071105910.3390/tropicalmed10070182