biostudies-literatureWu JNational Natural Science Foundation of ChinaOpen Fund of Yichang City Clinical Research Center for Mental Disorders821-831https://www.ebi.ac.uk/biostudies/studies/S-EPMC12301682biostudies-literatureUnknown22(7)<h4>Objective</h4>To examine the effect of Soyasaponin Bb on morphine withdrawal-induced anxiety-like behaviors in male mice and the corresponding mechanism.<h4>Methods</h4>The chronic intermittent escalating-dose morphine administration paradigm and spontaneous withdrawal were used to induce anxiety-like behaviors in male mice. Open field test (OFT) and elevated plus maze test (EPM) were used to evaluate anxiety-like behaviors. Soyasaponin Bb was intragastrically administered. Western blot was used to monitor the level of GSK-3β, Nrf2, and superoxidase dismutase in the basolateral amygdala (BLA). Virus-mediated gene overexpression and knockdown were used to manipulate the expression of GSK-3β and Nrf2 in the BLA. Two-way analysis of variance followed by Tukey's test were used to make compPsychiatry investigationSoyasaponin Bb Attenuates Morphine Withdrawal-Induced Anxiety-Like Behaviors in Male Mice by Rescuing GSK-3β/Nrf2 Signaling.PMC1230168281971248YCXL-23-1331200814Wu JLi ZLiu LLu YXiao ZShu YZhu CfalseSoyasaponin Bb Attenuates Morphine Withdrawal-Induced Anxiety-Like Behaviors in Male Mice by Rescuing GSK-3β/Nrf2 Signaling.<h4>Objective</h4>To examine the effect of Soyasaponin Bb on morphine withdrawal-induced anxiety-like behaviors in male mice and the corresponding mechanism.<h4>Methods</h4>The chronic intermittent escalating-dose morphine administration paradigm and spontaneous withdrawal were used to induce anxiety-like behaviors in male mice. Open field test (OFT) and elevated plus maze test (EPM) were used to evaluate anxiety-like behaviors. Soyasaponin Bb was intragastrically administered. Western blot was used to monitor the level of GSK-3β, Nrf2, and superoxidase dismutase in the basolateral amygdala (BLA). Virus-mediated gene overexpression and knockdown were used to manipulate the expression of GSK-3β and Nrf2 in the BLA. Two-way analysis of variance followed by Tukey's test were used to make comp2025-01-01T00:00:00Z2025 Jul2026-03-27T16:02:49.667Z2025-08-27T03:06:25.799ZS-EPMC123016824070847910.30773/pi.2025.0091