biostudies-literatureKobelyatskaya AAIDB RAS Government basic research programme49https://www.ebi.ac.uk/biostudies/studies/S-EPMC12302907biostudies-literatureUnknown18(1)<h4>Background</h4>Insulators are the multifunctional DNA binding proteins that perform architectural functions and regulate gene transcription. Although insulators have a well-established role in genome organization, it is still unclear how insulator proteins affect the control of tissue-specific processes. The Drosophila insulator BEAF32 (Boundary Element-Associated Factor of 32 kD) is a component of chromatin complexes found in open chromatin regions containing promoters of housekeeping genes. BEAF32 knockout impairs oogenesis and female fertility suggesting its specific functions during oogenesis.<h4>Results</h4>To get a better understanding of BEAF32 roles in oogenesis, we first examined its ovarian binding targets and discovered an enrichment of its localization sites in the promoterEpigenetics & chromatinInsulator BEAF32 regulates expression of tissue-specific genes and piRNA source loci in Drosophila ovaries.PMC12302907IDB RAS Government basic research programme 0088–2024–0010IDB RAS Government basic research programme 0088-2024-0010Kwon DAKobelyatskaya AAShchukina AAMorgunova VSokolova OFunikov SKalmykova AKalinkin AfalseInsulator BEAF32 regulates expression of tissue-specific genes and piRNA source loci in Drosophila ovaries.<h4>Background</h4>Insulators are the multifunctional DNA binding proteins that perform architectural functions and regulate gene transcription. Although insulators have a well-established role in genome organization, it is still unclear how insulator proteins affect the control of tissue-specific processes. The Drosophila insulator BEAF32 (Boundary Element-Associated Factor of 32 kD) is a component of chromatin complexes found in open chromatin regions containing promoters of housekeeping genes. BEAF32 knockout impairs oogenesis and female fertility suggesting its specific functions during oogenesis.<h4>Results</h4>To get a better understanding of BEAF32 roles in oogenesis, we first examined its ovarian binding targets and discovered an enrichment of its localization sites in the promoter2025-01-01T00:00:00Z2025 Jul2026-07-15T05:58:50.237Z2025-08-27T03:10:15.343ZS-EPMC123029074071708210.1186/s13072-025-00613-6