biostudies-literatureSchiemer RRCUK | Engineering and Physical Sciences Research Council (EPSRC)M.N.S. was supported by the National University of Malaysia (Universiti Kebangsaan Malaysia), Ministry of Higher Education MalaysiaR.S. was supported by Nottingham University Hospitals NHS Trust Clinical Research FellowshipRCUK | Engineering and Physical Sciences Research Council194-207https://www.ebi.ac.uk/biostudies/studies/S-EPMC12304263biostudies-literatureUnknown133(2)<h4>Background</h4>Endometrial cancer (EC) is the 6th most common cancer among women worldwide. No effective non-invasive screening methods or approved blood biomarkers for EC exist. Previous research explored Attenuated Total Reflection-Fourier Transform Infrared (ATR-FtIR) and Raman spectroscopies, using dried blood plasma. Fresh, 'wet', blood samples, that might provide faster results, have not been investigated. This study compared ATR-FtIR and Raman spectroscopies on 'wet' and dry blood plasma samples for EC detection. It also conducted a preliminary exploration into their diagnostic potential for EC in high-risk individuals with polycystic ovary syndrome (PCOS).<h4>Methods</h4>'Wet' and dry blood plasma samples from participants with EC, PCOS and healthy controls were analysed using British journal of cancerInfrared and Raman spectroscopy of blood plasma for rapid endometrial cancer detection.PMC12304263EP/W524402/1EP/L025620/1Schiemer RPhang SJones NWNotingher IGajjar KBShafiee MNBoitor RSeddon ABAtiomo WFurniss DGrant JfalseInfrared and Raman spectroscopy of blood plasma for rapid endometrial cancer detection.<h4>Background</h4>Endometrial cancer (EC) is the 6th most common cancer among women worldwide. No effective non-invasive screening methods or approved blood biomarkers for EC exist. Previous research explored Attenuated Total Reflection-Fourier Transform Infrared (ATR-FtIR) and Raman spectroscopies, using dried blood plasma. Fresh, 'wet', blood samples, that might provide faster results, have not been investigated. This study compared ATR-FtIR and Raman spectroscopies on 'wet' and dry blood plasma samples for EC detection. It also conducted a preliminary exploration into their diagnostic potential for EC in high-risk individuals with polycystic ovary syndrome (PCOS).<h4>Methods</h4>'Wet' and dry blood plasma samples from participants with EC, PCOS and healthy controls were analysed using 2025-01-01T00:00:00Z2025 Aug2026-03-27T17:10:07.203Z2025-08-30T03:06:25.578ZS-EPMC123042634038374010.1038/s41416-025-03050-0