biostudies-literatureVijaya Kumar DKCure Alzheimer's FundCure Alzheimer&apos;s FundDake Family FoundationNational Institutes of HealthNIH HHSe70490https://www.ebi.ac.uk/biostudies/studies/S-EPMC12307129biostudies-literatureUnknown21(8)<h4>Introduction</h4>Our previous studies demonstrated the antimicrobial properties of amyloid beta (Aβ) of Alzheimer's disease (AD) against clinically relevant bacteria, yeast, and viruses. In this study, we investigate the antimicrobial function of the 37-amino acid amylin of type 2 diabetes (T2D), expanding on its potential involvement in AD.<h4>Methods</h4>We used in vitro assays, including human three-dimensional neuronal cell culture models, to test microbicidal, microbiostatic, and synergistic antimicrobial interactions between amylin and Aβ against microbes.<h4>Results</h4>Our results confirm that amylin is a broad-spectrum antimicrobial peptide that exhibits both microbicidal and microbiostatic mechanisms. We also identified a synergistic antimicrobial effect between amylin and AβAlzheimer's & dementia : the journal of the Alzheimer's AssociationHuman amylin is a potent antimicrobial peptide that exhibits antimicrobial synergism with the amyloid beta protein.PMC123071292380231S10RR023594S10Mitchell TAMoir APSu CMoir RDKim ENavalpur Shanmugam NKChoi SHRodriguez ASVijaya Kumar DKEimer WATanzi RETailor BAGhelichi JfalseHuman amylin is a potent antimicrobial peptide that exhibits antimicrobial synergism with the amyloid beta protein.<h4>Introduction</h4>Our previous studies demonstrated the antimicrobial properties of amyloid beta (Aβ) of Alzheimer's disease (AD) against clinically relevant bacteria, yeast, and viruses. In this study, we investigate the antimicrobial function of the 37-amino acid amylin of type 2 diabetes (T2D), expanding on its potential involvement in AD.<h4>Methods</h4>We used in vitro assays, including human three-dimensional neuronal cell culture models, to test microbicidal, microbiostatic, and synergistic antimicrobial interactions between amylin and Aβ against microbes.<h4>Results</h4>Our results confirm that amylin is a broad-spectrum antimicrobial peptide that exhibits both microbicidal and microbiostatic mechanisms. We also identified a synergistic antimicrobial effect between amylin and Aβ2025-01-01T00:00:00Z2025 Aug2026-03-27T15:28:59.599Z2025-08-24T03:07:16.324ZS-EPMC123071294073118210.1002/alz.70490