biostudies-literatureZhao PNatural Science Foundation of Shandong Province (Shandong Provincial Natural Science Foundation)1124https://www.ebi.ac.uk/biostudies/studies/S-EPMC12307698biostudies-literatureUnknown8(1)Fibroblasts are abundant throughout the body and easily accessible without animal sacrifice. They possess the ability to proliferate and differentiate into muscle and fat tissues, as well as to produce extracellular matrix components, making them ideal for cultured meat production. In this study, we utilized the pig fibroblasts as seed cells and conducted myogenic/lipogenic transdifferentiation in 3D to create a muscle/fat matrix constituting whole-cut meat within 9 days. The muscle cells were efficiently derived from the fibroblasts overexpressing MyoD and the subsequent lipid deposition into the muscle was achieved by supplementing with a lipogenic inducer composed of olive oil and soybean lecithin. Lipidomic analysis revealed that the engineered meat exhibited a healthier fatty acid proCommunications biologyOptimizing fatty acids composition in meat-like tissue derived from myogenic conversion of pig fibroblasts.PMC12307698ZR2024MC096Lv JMi HZhao PGu WChen LCui HWang HKurdeko AfalseOptimizing fatty acids composition in meat-like tissue derived from myogenic conversion of pig fibroblasts.Fibroblasts are abundant throughout the body and easily accessible without animal sacrifice. They possess the ability to proliferate and differentiate into muscle and fat tissues, as well as to produce extracellular matrix components, making them ideal for cultured meat production. In this study, we utilized the pig fibroblasts as seed cells and conducted myogenic/lipogenic transdifferentiation in 3D to create a muscle/fat matrix constituting whole-cut meat within 9 days. The muscle cells were efficiently derived from the fibroblasts overexpressing MyoD and the subsequent lipid deposition into the muscle was achieved by supplementing with a lipogenic inducer composed of olive oil and soybean lecithin. Lipidomic analysis revealed that the engineered meat exhibited a healthier fatty acid pro2025-01-01T00:00:00Z2025 Jul2026-03-18T14:04:45.041Z2025-08-23T03:07:04.185ZS-EPMC123076984073089110.1038/s42003-025-08574-y