{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Johnson RM"],"funding":["U.S. Department of Health & Human Services | NIH | National Center for Advancing Translational Sciences","Department of Education and Training | Australian Research Council (ARC)","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NCATS NIH HHS","Department of Education and Training | Australian Research Council","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)","NIAID NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)","National Science Foundation (NSF)","U.S. Department of Health & Human Services | National Institutes of Health (NIH)","Ambrose Monell Foundation","Trumbull College Richter Fellowship","NIGMS NIH HHS","U.S. Department of Health & Human Services | National Institutes of Health","National Science Foundation"],"pagination":["6971"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12307751"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Wolbachia is a promising strategy to inhibit dengue virus (DENV) transmission by Ae. aegypti mosquitoes. Laboratory studies assessing DENV inhibition by Wolbachia typically have not considered natural frequent mosquito blood feeding behavior. Here, we determine the impact of successive feeding on DENV-2 transmission by Ae. aegypti in the presence or absence of Wolbachia (wAlbB and wMelM strains). We show that successive feeding shortens the extrinsic incubation period (EIP) in wildtype (WT; without Wolbachia) and wAlbB mosquitoes through enhanced dissemination. Feeding empirical data into models showed that successive feeding increases the probability of WT and wAlbB mosquitoes surviving beyond the EIP. Importantly, the more epidemiologically relevant comparison of the odds of wAlbB mosquitoes surviving beyond the EIP relative to WT, reveals a larger impact of successive feeding on WT than wAlbB. This indicates a strong inhibitory effect of Wolbachia even in the context of natural frequent mosquito blood feeding behavior."],"journal":["Nature communications"],"pubmed_title":["Implications of successive blood feeding on Wolbachia-mediated dengue virus inhibition in Aedes aegypti mosquitoes."],"pmcid":["PMC12307751"],"funding_grant_id":["AI148477","R35GM143029","T32 AI055403","DE230100067","DGE-2139841","R35 GM143029","R01 AI148477","T32AI055403","UL1 TR001863"],"pubmed_authors":["Ott IM","Grubaugh ND","Vogels CBF","Sodeinde A","Gu X","Breban MI","Nolan BL","Ross PA","Brackney DE","Johnson RM","Perkins TA"],"additional_accession":[]},"is_claimable":false,"name":"Implications of successive blood feeding on Wolbachia-mediated dengue virus inhibition in Aedes aegypti mosquitoes.","description":"Wolbachia is a promising strategy to inhibit dengue virus (DENV) transmission by Ae. aegypti mosquitoes. Laboratory studies assessing DENV inhibition by Wolbachia typically have not considered natural frequent mosquito blood feeding behavior. Here, we determine the impact of successive feeding on DENV-2 transmission by Ae. aegypti in the presence or absence of Wolbachia (wAlbB and wMelM strains). We show that successive feeding shortens the extrinsic incubation period (EIP) in wildtype (WT; without Wolbachia) and wAlbB mosquitoes through enhanced dissemination. Feeding empirical data into models showed that successive feeding increases the probability of WT and wAlbB mosquitoes surviving beyond the EIP. Importantly, the more epidemiologically relevant comparison of the odds of wAlbB mosquitoes surviving beyond the EIP relative to WT, reveals a larger impact of successive feeding on WT than wAlbB. This indicates a strong inhibitory effect of Wolbachia even in the context of natural frequent mosquito blood feeding behavior.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-03-31T11:55:30.109Z","creation":"2025-09-01T03:08:30.518Z"},"accession":"S-EPMC12307751","cross_references":{"pubmed":["40730791"],"doi":["10.1038/s41467-025-62352-2"]}}