biostudies-literatureUnknown15(1)Reichelt-Wurm SUniversitätsklinikum RegensburgThe hypoxia-inducible factor 1α (Hif1α) represents the master transcription factor coordinating cellular responses to oxygen depletion. With hundreds of target genes it plays a key role in numerous bio-medical conditions as well as neoplastic and non-cancerous diseases, which in turn requires a strict regulation. Long non-coding RNAs have the potential to virtually control every step of gene expression. We aimed to investigate the expression and role of HIF1A antisense lncRNAs HIF1A-AS1, AS2, and AS3 under hyperglycemic, hypoxic, or both conditions in three non-cancerous human renal cell types: HK-2 cells, primary RPTECs, and mesangial cells. We observed that HIF1A-AS2 and AS3 expression was upregulated under oxygen deprivation. Furthermore, knockdown (KD) of HIF1A-AS3 resulted in a signifScientific reports27876https://www.ebi.ac.uk/biostudies/studies/S-EPMC12310967biostudies-literatureCell-type specific effects of the long non-coding RNA HIF1A-AS3 on HIF1A expression in kidney cells.PMC12310967Kretz MReichelt-Wurm SKnauss LEggenhofer EScharf MStrasser BHoller KBanas BBanas MCfalseCell-type specific effects of the long non-coding RNA HIF1A-AS3 on HIF1A expression in kidney cells.The hypoxia-inducible factor 1α (Hif1α) represents the master transcription factor coordinating cellular responses to oxygen depletion. With hundreds of target genes it plays a key role in numerous bio-medical conditions as well as neoplastic and non-cancerous diseases, which in turn requires a strict regulation. Long non-coding RNAs have the potential to virtually control every step of gene expression. We aimed to investigate the expression and role of HIF1A antisense lncRNAs HIF1A-AS1, AS2, and AS3 under hyperglycemic, hypoxic, or both conditions in three non-cancerous human renal cell types: HK-2 cells, primary RPTECs, and mesangial cells. We observed that HIF1A-AS2 and AS3 expression was upregulated under oxygen deprivation. Furthermore, knockdown (KD) of HIF1A-AS3 resulted in a signif2025-01-01T00:00:00Z2025 Jul2026-07-15T09:43:49.61Z2025-08-16T03:07:07.942ZS-EPMC123109674073916210.1038/s41598-025-12441-5