{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fuller CN"],"funding":["NIGMS NIH HHS"],"pagination":["13965-13973"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12312367"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["97(26)"],"pubmed_abstract":["Ultra-high-resolution Fourier transform ion cyclotron resonance mass spectrometry (UHR FT-ICR MS) for top-down proteomics has shown the potential to resolve proteoforms and splice variants, particularly those with post-translational modifications. Here, we integrate trapped ion mobility and mass selection in tandem with ultraviolet photodissociation (UVPD) followed by FT-ICR MS measurements. The proposed method using mobility/mass-selected UVPD before FT-ICR MS allows for high protein sequence coverage and PTM localization with high mass accuracy (<1 ppm) and a better duty cycle (2×). When applied to the analysis of a bovine histone mixture, characteristic UVPD <i>a</i>/<i>b</i>/<i>c</i>/<i>x</i>/<i>y</i>/<i>z</i> ions led to the annotation of 51 proteoforms from H2B, H2A, and H4 core hist"],"journal":["Analytical chemistry"],"pubmed_title":["Histone Analysis Using Mobility- and Mass-Selected Ultraviolet Photodissociation in Tandem with Ultra-High-Resolution Mass Spectrometry."],"pmcid":["PMC12312367"],"funding_grant_id":["R35 GM153450"],"pubmed_authors":["Fuller CN","Kosmopoulou M","Jeanne Dit Fouque K","Valadares Tose L","Alam MS","Fernandez-Lima F","Van Orden S","Garcia BA","Searfoss RM","de Luna Vitorino FN","Ridgeway ME","Suckau D"],"additional_accession":[]},"is_claimable":false,"name":"Histone Analysis Using Mobility- and Mass-Selected Ultraviolet Photodissociation in Tandem with Ultra-High-Resolution Mass Spectrometry.","description":"Ultra-high-resolution Fourier transform ion cyclotron resonance mass spectrometry (UHR FT-ICR MS) for top-down proteomics has shown the potential to resolve proteoforms and splice variants, particularly those with post-translational modifications. Here, we integrate trapped ion mobility and mass selection in tandem with ultraviolet photodissociation (UVPD) followed by FT-ICR MS measurements. The proposed method using mobility/mass-selected UVPD before FT-ICR MS allows for high protein sequence coverage and PTM localization with high mass accuracy (<1 ppm) and a better duty cycle (2×). When applied to the analysis of a bovine histone mixture, characteristic UVPD <i>a</i>/<i>b</i>/<i>c</i>/<i>x</i>/<i>y</i>/<i>z</i> ions led to the annotation of 51 proteoforms from H2B, H2A, and H4 core hist","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul","modification":"2026-03-18T13:31:41.622Z","creation":"2025-08-18T09:54:19.408Z"},"accession":"S-EPMC12312367","cross_references":{"pubmed":["40552939"],"doi":["10.1021/acs.analchem.5c02166"]}}