biostudies-literatureChatziantoniou CNIHR Biomedical Research Centre, Royal Marsden NHS Foundation Trust/Institute of Cancer ResearchStichting Kinderen KankervrijRoyal Marsden Cancer Charity1598-1610https://www.ebi.ac.uk/biostudies/studies/S-EPMC12321683biostudies-literatureUnknown55(8)<h4>Background</h4>The apparent diffusion coefficient (ADC) derived from diffusion-weighted imaging (DWI) is a potential biomarker for treatment response in pediatric rhabdomyosarcoma. Due to its rarity, investigations into this marker require multicenter approaches, which can result in variability in acquisition parameters.<h4>Objective</h4>To evaluate the impact of different acquisition parameters on ADC estimates in a multicenter dataset of rhabdomyosarcoma patients.<h4>Materials and methods</h4>We included 114 pediatric and adolescent rhabdomyosarcoma patients from 22 treatment centers (195 scans). Median age: 6.0 years (0.3-21.8). We evaluated the impact of voxel size, (number of) b-values, and echo time on tumor ADC values. The effect of the highest b-value was separately investigatePediatric radiologyAcquisition matters - how do scan parameters affect apparent diffusion coefficient estimates in pediatric rhabdomyosarcoma.PMC12321683357Leseur JDi Paolo PLKlein WMRuggiero ASirvent SIOrbach DGlosli HMinard-Colin VCaro-Dominguez PPerruccio KGiraudo CMorosi CPace EFerrari ARenard MMandeville HCvan Rijn RRQuaglietta LBertolini PBisogno GDandis RFiocco MDe Luca ADevalck CLeemans AComa AJenney MChatziantoniou CMoalla Svan Ewijk RMcHugh KAdams MSchoot RADe Donno GTer Horst SAJCharon VMerks JHMOrding Muller LSGallego SBouhamama AfalseAcquisition matters - how do scan parameters affect apparent diffusion coefficient estimates in pediatric rhabdomyosarcoma.<h4>Background</h4>The apparent diffusion coefficient (ADC) derived from diffusion-weighted imaging (DWI) is a potential biomarker for treatment response in pediatric rhabdomyosarcoma. Due to its rarity, investigations into this marker require multicenter approaches, which can result in variability in acquisition parameters.<h4>Objective</h4>To evaluate the impact of different acquisition parameters on ADC estimates in a multicenter dataset of rhabdomyosarcoma patients.<h4>Materials and methods</h4>We included 114 pediatric and adolescent rhabdomyosarcoma patients from 22 treatment centers (195 scans). Median age: 6.0 years (0.3-21.8). We evaluated the impact of voxel size, (number of) b-values, and echo time on tumor ADC values. The effect of the highest b-value was separately investigate2025-01-01T00:00:00Z2025 Jul2025-08-30T03:15:48.601Z2025-08-30T03:09:22.015ZS-EPMC123216834049304810.1007/s00247-025-06263-w